胡桃科微卫星位点的分离与鉴定
Isolation and characterization of microsatellite loci from (Juglandaceae).
作者信息
Wang Peng-Fei, Li Yong, Qian Zhi-Hao, Li Jia-Xin, Ge Xue-Jun
机构信息
Innovation Platform of Molecular Biology College of Forestry Henan Agricultural University Zhengzhou 450002 People's Republic of China.
South China Botanical Garden Chinese Academy of Sciences Guangzhou 510650 People's Republic of China.
出版信息
Appl Plant Sci. 2018 Dec 5;6(12):e01205. doi: 10.1002/aps3.1205. eCollection 2018 Dec.
PREMISE OF THE STUDY
Microsatellite markers of (Juglandaceae) were developed for future studies on the population genetic diversity and spatial genetic structure of the species.
METHODS AND RESULTS
Based on Illumina sequencing of the transcriptome of , a total of 2452 microsatellites were identified from 83,674 assembled unigenes. One hundred microsatellites were randomly selected to design amplification primer pairs. Of these, 15 were successfully amplified and displayed polymorphism. For these markers, the number of alleles per locus and population ranged from one to six. The levels of observed and expected heterozygosity varied from 0.000 to 1.000 and 0.000 to 0.718, respectively. Furthermore, all of the 15 loci were successfully cross-amplified in another congeneric species () and were demonstrated to be polymorphic.
CONCLUSIONS
The microsatellite loci described here can be used for future population genetic and landscape genetic studies on .
研究前提
开发了胡桃科（Juglandaceae）的微卫星标记，用于该物种未来的种群遗传多样性和空间遗传结构研究。
方法与结果
基于对某物种转录组的Illumina测序，从83,674个组装的单基因中总共鉴定出2452个微卫星。随机选择100个微卫星设计扩增引物对。其中，15个成功扩增并表现出多态性。对于这些标记，每个位点和群体的等位基因数从1到6不等。观察到的杂合度和预期杂合度水平分别从0.000到1.000和0.000到0.718不等。此外，所有15个位点在另一个同属物种中成功进行了交叉扩增，并被证明具有多态性。
结论
这里描述的微卫星位点可用于该物种未来的种群遗传学和景观遗传学研究。
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