Laboratory of Subtropical Biodiversity, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, People's Republic of China.
Appl Plant Sci. 2013 May 7;1(6). doi: 10.3732/apps.1200524. eCollection 2013 Jun.
Microsatellite loci were developed for a monotypic species endemic to subtropical China, Cyclocarya paliurus, to help infer the evolutionary histories of ancient monotypic genera in subtropical China. •
Using the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol, 28 primer sets were identified in two wild populations. All loci were polymorphic, with the number of alleles per locus ranging from two to eight. The expected and observed heterozygosities ranged from 0.153 to 0.802 and from 0 to 0.750, respectively. The transferability of the 28 primer pairs was tested on Juglans regia, Pterocarya stenoptera, and Platycarya strobilacea. Twenty-one (75.0%), 22 (78.6%), and 15 (53.6%) markers were successfully amplified in J. regia, P. stenoptera, and P. strobilacea, respectively. •
These loci will be useful for in-depth analysis of genetic diversity and phylogeographical variation throughout the distribution range of C. paliurus.
为中国亚热带特有单种属圆齿火棘(Cyclocarya paliurus)开发微卫星位点,以帮助推断中国亚热带古单种属的进化历史。
使用快速 AFLP 分离序列中的重复(FIASCO)方案,在两个野生种群中鉴定出 28 对引物。所有位点均具有多态性,每个位点的等位基因数范围为 2 到 8。期望和观察杂合度分别为 0.153 到 0.802 和 0 到 0.750。28 对引物对的可转移性在胡桃、青钱柳和枫杨上进行了测试。在胡桃、青钱柳和枫杨中,分别有 21 个(75.0%)、22 个(78.6%)和 15 个(53.6%)标记成功扩增。
这些位点将有助于深入分析圆齿火棘分布范围内的遗传多样性和系统地理变异。