Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Departamento de Microbiología, Inmunología, Biotecnología y Genética, Cátedra de Inmunología, IDEHU-CONICET, Ciudad de Buenos Aires, Argentina.
Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Departamento de Sanidad, Nutrición, Bromatología y Toxicología, Cátedra de Nutrición, Ciudad de Buenos Aires, Argentina.
Nutrition. 2019 Apr;60:161-169. doi: 10.1016/j.nut.2018.10.024. Epub 2018 Oct 24.
We aimed to analyze the effect of a protein-deficient diet on mucosal and systemic immunity during a Trichinella spiralis infection.
Two groups of weaning Wistar rats received a protein-deficient diet (6.5% casein) and the other two groups received a control diet (20% casein). After 10 d, one group of each diet was infected (PD and C) with muscle larvae (infecting stage). Food intake and body weight were assessed over time. Blood eosinophils counts, antibodies in serum, and tissue extracts were assessed at different days postinfection. Histologic studies were done in the lungs and intestines, and adult worm (AW) fecundity index score and muscle parasite burden were determined.
Food and protein intake were lower in PD than in C Body weight was lower in PD than in a non-infected protein-deficient diet. Eosinophils counts were lower in PD than in C Total and specific antibodies were lower in PD than C PD had a reduced number of mast and goblet cells in the lungs and intestines compared with C The persistence of AW in the intestines and migrant larvae at the lungs was longer in PD than in C. The AW fecundity index score was higher in PD than in C. Finally, PD evidenced a higher muscular parasite burden than C.
Protein deficiency affects the mucosal and systemic immune response to Trichinella spiralis and delays the expulsion and increases the fecundity index score of AW, which leads to a higher parasite burden in the muscles.
分析在旋毛虫感染期间，蛋白质缺乏饮食对黏膜和全身免疫的影响。
两组断奶 Wistar 大鼠分别接受蛋白质缺乏饮食（6.5%酪蛋白）和对照组饮食（20%酪蛋白）。10d 后，每组的一个饮食组接受肌肉幼虫（感染阶段）感染（PD 和 C）。随时间评估食物摄入量和体重。在不同的感染后天数评估血清中的血液嗜酸性粒细胞计数、抗体和组织提取物。在肺部和肠道进行组织学研究，并确定成虫（AW）生殖指数评分和肌肉寄生虫负荷。
PD 组的食物和蛋白质摄入量低于 C 组，体重低于非感染性蛋白质缺乏饮食的 C 组。PD 组的嗜酸性粒细胞计数低于 C 组。PD 组的总抗体和特异性抗体均低于 C 组。与 C 组相比，PD 组肺部的肥大细胞和杯状细胞数量减少，肠道中的 AW 滞留时间更长，肠道中的迁移幼虫。PD 的 AW 生殖指数评分高于 C 组。最后，PD 组肌肉寄生虫负荷高于 C 组。
蛋白质缺乏会影响旋毛虫黏膜和全身免疫反应，并延迟 AW 的排出，增加 AW 的生殖指数评分，导致肌肉寄生虫负荷增加。
