Applied Biochemistry Lab, Department of Molecular Biology and Biotechnology, Tezpur University, Napaam, Sonitpur, Assam, India.
J Basic Microbiol. 2019 Mar;59(3):238-248. doi: 10.1002/jobm.201800298. Epub 2019 Jan 2.
A biosurfactant, produced by Achromobacter sp. TMB1 is reported through this investigation having physiochemical properties useful for operational and remedial activities in petroleum industries. The strain isolated from soils of local petrol pumps when allowed to grow in glucose containing mineral salts media (MSM) in ambient environment that is, 30 °C and varying pH in between 5.5 and 7.2, showed surface tension reduction as low as 34.25 dyne cm at an interval of 48-72 h. Taguchi experiment confirmed the contribution of glucose, yeast extracts, and NaNO concentration on biosurfactant production. FTIR and H NMR analysis of culture supernatant obtained after acid precipitation and solvent extraction methods revealed the glycolipid nature of the extracted compounds. HR-LCMS study further revealed the presence of 10 different types of mono- and di-rhamnolipids congeners with the fatty acids carbon length C . The isolated biosurfactant shows functional stability in the temperature range 20-100 °C and pH range 2-12 while maintaining the structural integrity till 550 °C (TGA-DSC), strongly suggests its potential applications in petroleum industries.
一种生物表面活性剂，由阿克默obacter sp. TMB1 产生，通过本研究报告具有在石油工业的操作和补救活动中有用的物理化学性质。从当地加油站的土壤中分离出来的菌株，当在含有葡萄糖的无机盐培养基（MSM）中在环境温度（即 30°C）和 pH 值在 5.5 到 7.2 之间变化的环境中生长时，显示出低至 34.25 达因/厘米的表面张力降低，间隔 48-72 小时。田口实验证实了葡萄糖、酵母提取物和 NaNO 浓度对生物表面活性剂生产的贡献。酸沉淀和溶剂萃取法获得的培养上清液的 FTIR 和 H NMR 分析表明，提取化合物具有糖脂的性质。HR-LCMS 研究进一步表明，存在 10 种不同类型的单和二鼠李糖脂同系物，其脂肪酸碳长 C 。分离的生物表面活性剂在 20-100°C 的温度范围和 2-12 的 pH 值范围保持功能稳定性，同时在 550°C（TGA-DSC）下保持结构完整性，强烈表明其在石油工业中的潜在应用。
