Haloi Saurav, Medhi Tapas
Applied Biochemistry Lab, Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur, Assam India.
Indian J Microbiol. 2022 Sep;62(3):434-440. doi: 10.1007/s12088-022-01021-0. Epub 2022 Apr 25.
Rhamnolipid producing was selected for this investigation to optimize the metabolite production in fabricated batch reactor after studying yield kinetics in shake-flask and tried to reduce the overall production cost through recovery technology. Using various kinetic models, maximum specific growth rate (μ) and half velocity constant (K) of were determined to be 0.185 ± 0.0025 h and 0.124 ± 0.024 g/L in shake-flask, respectively. Further, a batch reactor was designed with integration of a foam fractionate column in the lid of the vessel and their performances were compared with shake-flask studies. The yields of rhamnolipids production on biomass (Y), rhamnolipids production on substrate (Y) and biomass production on substrate (Y) were found to be higher in reactor than that of shake-flask. The best conditions for maximum rhamnolipid production in reactor were observed to be 2 vvm and 300 rpm, giving Y = 0.152 g/g, Y = 0.542 g/g and Y = 0.280 g/g. Rhamnolipid production was increased by ≈ 10.18% in the reactor than that of shake-flask in optimized conditions. Rhamnolipid concentrations in the foamate were also found to be higher than that of reactor vessels. Further, the performance of foam fractionation was validated through enrichment and recovery, which were found in the range of 2.75-4.86 and 25.33-64.64%, respectively.
The online version contains supplementary material available at 10.1007/s12088-022-01021-0.
在摇瓶中研究产量动力学后，选择产鼠李糖脂的菌株用于本研究，以优化在定制分批反应器中的代谢产物产量，并试图通过回收技术降低总体生产成本。使用各种动力学模型，在摇瓶中测定的最大比生长速率（μ）和半速度常数（K）分别为0.185±0.0025 h⁻¹和0.124±0.024 g/L。此外，设计了一个分批反应器，在容器盖上集成了一个泡沫分馏柱，并将其性能与摇瓶研究进行了比较。发现反应器中鼠李糖脂产量对生物量（Y）、鼠李糖脂产量对底物（Y）和生物量产量对底物（Y）的产率均高于摇瓶。观察到反应器中最大鼠李糖脂产量的最佳条件为2 vvm和300 rpm，此时Y = 0.152 g/g，Y = 0.542 g/g，Y = 0.280 g/g。在优化条件下，反应器中的鼠李糖脂产量比摇瓶提高了约10.18%。还发现泡沫产物中的鼠李糖脂浓度高于反应器容器中的浓度。此外，通过富集和回收验证了泡沫分馏的性能，富集和回收范围分别为2.75 - 4.86%和25.33 - 64.64%。
在线版本包含可在10.1007/s12088 - 022 - 01021 - 0获取的补充材料。
