Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
School of Future Technology, University of Chinese Academy of Sciences, Beijing, 100190, China.
Angew Chem Int Ed Engl. 2019 Mar 18;58(12):3834-3837. doi: 10.1002/anie.201813239. Epub 2019 Feb 14.
Cryosurgery has attracted much attention for the treatment of tumors owing to its clear advantages. However, determining the volume of frozen tissues in real-time remains a challenge, which greatly lowers the therapeutic efficacy of cryosurgery and hinders its broad application for the treatment of cancers. Herein, we report a freezing-induced turn-on strategy for the selective real-time imaging of frozen cancer cells. As a type of aggregation-induced emission (AIE) fluorogen, TABD-Py molecules interact specifically with ice crystals and form aggregates at the ice/water interface. Consequently, bright fluorescent emission appears upon freezing. TABD-Py molecules are enriched mostly in the cancer cells and exhibit high biocompatibility as well as low cytotoxicity; therefore, a freezing-induced turn-on imaging modality for cryosurgery is developed, which will certainly maximize the therapeutic efficacy of cryosurgery in treating tumors.
冷冻疗法因其明显的优势而备受关注，有望用于肿瘤的治疗。然而，实时确定冷冻组织的体积仍然是一个挑战，这极大地降低了冷冻疗法的疗效，限制了其在癌症治疗中的广泛应用。在此，我们报道了一种基于冷冻诱导开启的策略，用于选择性实时成像冷冻的癌细胞。作为一种聚集诱导发光（AIE）荧光团，TABD-Py 分子特异性地与冰晶相互作用，并在冰/水界面形成聚集体。因此，在冷冻时会出现明亮的荧光发射。TABD-Py 分子主要富集在癌细胞中，表现出高生物相容性和低细胞毒性；因此，开发了一种用于冷冻疗法的冷冻诱导开启成像模式，这必将最大限度地提高冷冻疗法治疗肿瘤的疗效。
