Ho Wei-Ting, Chang Jung-Shen, Chen Tsan-Chi, Chou San-Fang, Wang I-Jong, Chang Shu-Wen
Department of Ophthalmology, Far Eastern Memorial Hospital, New Taipei City, Taiwan.
Graduate Institute of Clinical Medicine, National Taiwan University, Taipei, Taiwan.
Cornea. 2019 Mar;38(3):360-363. doi: 10.1097/ICO.0000000000001833.
To evaluate the safety and feasibility of patent blue (PB) as the vital dye in Descemet membrane endothelial keratoplasty (DMEK).
Bovine corneal endothelial cells were incubated with different concentrations (0.02%-2.5%) of PB. The cell viability, which was assessed by Cell Counting Kit-8 assay, was compared with that of untreated control and 0.06% to 0.4% trypan blue. The dyes were also used for graft preparation and implantation in the porcine eye model to evaluate stain quality, dye retention, and the feasibility of using PB in DMEK surgery.
No obvious increase in cytotoxicity was detected for 0.06% to 0.4% trypan blue and PB at concentrations up to 1.0%, but the cell viability after incubating with 1.5% to 2.5% PB was significantly reduced. PB at 0.5% to 1.0% generated good staining quality that can be used to facilitate graft implantation. Although the staining quality of 0.5% to 1.0% PB faded to an intermediate level after a 30-minute wash in phosphate-buffered saline, dye retention persisted for up to 24 hours.
PB at 0.5% to 1.0% is biocompatible and can stain the graft sufficiently, making it an alternative for DMEK surgery.
评估专利蓝（PB）作为活体染料在Descemet膜内皮角膜移植术（DMEK）中的安全性和可行性。
将牛角膜内皮细胞与不同浓度（0.02%-2.5%）的PB孵育。通过细胞计数试剂盒-8法评估细胞活力，并与未处理的对照组以及0.06%至0.4%的台盼蓝进行比较。这些染料还用于猪眼模型的移植物制备和植入，以评估染色质量、染料保留情况以及PB在DMEK手术中的可行性。
0.06%至0.4%的台盼蓝以及浓度高达1.0%的PB未检测到明显的细胞毒性增加，但与1.5%至2.5%的PB孵育后细胞活力显著降低。0.5%至1.0%的PB产生了良好的染色质量，可用于促进移植物植入。尽管在磷酸盐缓冲盐水中洗涤30分钟后，0.5%至1.0%的PB染色质量褪色至中等水平，但染料保留可持续长达24小时。
0.5%至1.0%的PB具有生物相容性，能够充分染色移植物，使其成为DMEK手术的一种替代选择。
