Centre for Ophthalmology, University of Tuebingen, Tuebingen, Germany.
J Cataract Refract Surg. 2011 Jun;37(6):1147-53. doi: 10.1016/j.jcrs.2010.12.051.
To evaluate capsule-staining properties and biocompatibility of the triarylmethane dyes methyl blue and aniline blue compared with patent blue and trypan blue on cultured human corneal endothelial cells.
Centre for Ophthalmology, University of Tuebingen, Tuebingen, Germany.
Experimental study.
Human corneal endothelial cell cultures were harvested from human donor cells and exposed to various concentrations (0.025 to 5.0 mg/mL) of methyl blue, aniline blue, patent blue, and trypan blue. Cytotoxicity was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide test after 24 hours of incubation. Calcein live cell staining was performed at the same time point. The dyes were also used to stain pig lens capsules in vitro by incubating the lenses for 1 minute with 3 concentrations (0.5, 1.5, and 2.5 mg/mL) of dye, after which the staining properties were evaluated.
No significant cytotoxicity was detected for patent blue and methyl blue at any tested concentration. However, aniline blue exerted significant cytotoxicity at concentrations of 1.5 mg/mL or higher and trypan blue at 2.5 mg/mL or higher. Capsule staining of the tested triarylmethane dyes was suitable for performing capsulorhexis, but only at higher concentrations than with trypan blue.
High concentrations and long incubation times of trypan blue and aniline blue showed significant cytotoxicity to human cultured endothelial cells in contrast to patent blue and methyl blue. All tested dyes were able to stain lens capsules sufficiently for capsulorhexis creation.
No author has a financial or proprietary interest in any material or method mentioned.
评估三芳甲烷染料亚甲蓝和苯胺蓝与专利蓝和锥虫蓝在培养的人角膜内皮细胞上的胶囊染色特性和生物相容性。
德国图宾根大学眼科中心。
实验研究。
从人供体细胞中收获人角膜内皮细胞培养物，并将其暴露于不同浓度（0.025 至 5.0mg/mL）的亚甲蓝、苯胺蓝、专利蓝和锥虫蓝。孵育 24 小时后通过 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐试验评估细胞毒性。同时进行 calcein 活细胞染色。还通过用 3 种浓度（0.5、1.5 和 2.5mg/mL）的染料孵育晶状体 1 分钟，然后评估染色特性，在体外用这些染料对猪晶状体囊进行染色。
在任何测试浓度下，专利蓝和亚甲蓝均未显示出明显的细胞毒性。然而，在 1.5mg/mL 或更高浓度下，苯胺蓝表现出明显的细胞毒性，而在 2.5mg/mL 或更高浓度下，锥虫蓝表现出明显的细胞毒性。所测试的三芳甲烷染料的囊染色适用于进行囊切开术，但仅在高于锥虫蓝的浓度下。
与专利蓝和亚甲蓝相比，锥虫蓝和苯胺蓝的高浓度和长时间孵育对人培养的内皮细胞表现出明显的细胞毒性。所有测试的染料都能够充分染色晶状体囊，以进行囊切开术。
没有作者在任何材料或方法中拥有财务或专有权益。
