Ploskonos M V
Klin Lab Diagn. 2016 Jun;61(6):342-7.
The article presents description of isolation of spermatozoa from native ejaculate: technique of washing off seminal plasma. technique of swim-up and technique of centrifugation of ejaculate in density gradient. The comparative characteristic of effectiveness of techniques is given. The shortcomings of techniques are described too. The choice of technique depends on necessity of carrying out isolation of germ cells for investigation purposes or clinical application and also on characteristics of ejaculate and rules specified in particular laboratory.
冲洗精浆的技术、上游法和密度梯度离心射精技术。给出了这些技术有效性的比较特征。还描述了这些技术的缺点。技术的选择取决于为研究目的或临床应用而进行生殖细胞分离的必要性,也取决于射精的特征以及特定实验室规定的规则。