碘克沙醇作为用于分离活动精子的密度梯度介质。
Iodixanol as a density gradient medium for the isolation of motile spermatozoa.
作者信息
Harrison K
机构信息
Queensland Fertility Group, Brisbane, Australia.
出版信息
J Assist Reprod Genet. 1997 Aug;14(7):385-7. doi: 10.1007/BF02766145.
PURPOSE
The purpose of this study was to establish concentrations of Iodixanol which could be used in a similar manner to the widely used Mini-Percoll technique for the separation of motile spermatozoa.
METHODS
Various density gradient combinations of Iodixanol were compared for the isolation of motile spermatozoa from cryopreserved donor semen. The toxicity of Iodixanol was tested by its effect on the growth of two-cell mouse embryos and fresh sperm survival.
RESULTS
The best sperm recovery (32%) came from the pellet of sperm passing through a discontinuous gradient of 25% over 40% OptiPrep after 20 min of centrifugation at 400 g. There was no evidence of toxicity to mouse embryo growth or sperm survival.
CONCLUSIONS
Iodixanol (OptiPrep; Nycomed) provides a satisfactory alternative for the efficient separation of motile spermatozoa for ART procedures.
目的
本研究的目的是确定碘克沙醇的浓度，其可用于以与广泛使用的小体积 Percoll 技术类似的方式分离活动精子。
方法
比较了碘克沙醇的各种密度梯度组合，用于从冷冻保存的供体精液中分离活动精子。通过碘克沙醇对二细胞小鼠胚胎生长和新鲜精子存活的影响来测试其毒性。
结果
在 400 g 离心 20 分钟后，最佳精子回收率（32%）来自穿过 25% 覆盖 40% OptiPrep 的不连续梯度的精子沉淀。没有证据表明对小鼠胚胎生长或精子存活有毒性。
结论
碘克沙醇（OptiPrep；Nycomed）为辅助生殖技术程序中有效分离活动精子提供了令人满意的替代方法。
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