Université de Lyon, Université Claude Bernard Lyon 1, CNRS UMR-5310, INSERM U-1217, Institut NeuroMyoGène, F-69008 Lyon, France.
Mol Biol Cell. 2019 Mar 1;30(5):636-645. doi: 10.1091/mbc.E18-07-0429. Epub 2019 Jan 2.
Producing mature spermatozoa is essential for sexual reproduction in metazoans. Spermiogenesis involves dramatic cell morphological changes going from sperm tail elongation and nuclear reshaping to cell membrane remodeling during sperm individualization and release. The sperm manchette plays a critical scaffolding function during nuclear remodeling by linking the nuclear lamina to the cytoskeleton. Here, we describe the role of an uncharacterized protein in Drosophila, salto/CG13164, involved in nuclear shaping and spermatid individualization. Salto has dynamic localization during spermatid differentiation, being progressively relocated from the sperm-nuclear dense body, which is equivalent to the mammalian sperm manchette, to the centriolar adjunct and acrosomal cap during spermiogenesis. salto-null male flies are sterile and exhibit complete spermatid individualization defects. salto-deficient spermatids show coiled spermatid nuclei at late maturation stages and stalled individualization complexes. Our work sheds light on a novel component involved in cytoskeleton-based cell-morphological changes during spermiogenesis.
产生成熟的精子对于后生动物的有性繁殖至关重要。精子发生包括从精子尾部伸长和核重塑到精子个体化和释放过程中的细胞膜重塑的显著细胞形态变化。精子套索在核重塑过程中通过将核纤层与细胞骨架连接起来发挥关键的支架功能。在这里，我们描述了果蝇中一个未被表征的蛋白质 salto/CG13164 在核形成和精细胞个体化中的作用。Salto 在精细胞分化过程中的定位具有动态性，从相当于哺乳动物精子套索的精子核致密体逐渐重新定位到中心粒附属物和顶体帽。salto 缺失的雄性苍蝇不育，并表现出完全的精细胞个体化缺陷。salto 缺陷的精细胞在晚期成熟阶段显示卷曲的精细胞核和停滞的个体化复合物。我们的工作揭示了一个参与精子发生过程中基于细胞骨架的细胞形态变化的新组件。
