Kosykh V G, Mantsygin Iu A, Sviatukhina N V, Vitenene I V, Bur'ianov Ia I
Biokhimiia. 1988 Sep;53(9):1474-8.
Monoclonal antibodies against EcoRII endonuclease were obtained after immunization of two BALB/c mice with a homogeneous enzyme prepared by conventional methods. IgG from ascitic fluid was purified and coupled to CNBr-activated Sepharose 4B to give a specific column used to isolate EcoRII endonuclease. The isolated EcoRII endonuclease produced a single band during SDS gel electrophoresis.
