Kosykh V G, Puntezhis S A, Bur'ianov Ia I, Baev A A
Biokhimiia. 1982 Apr;47(4):619-25.
The restriction endonuclease Eco RII was isolated and purified to homogeneity. The isolation procedure involved the use of the E. coli strain B834/pSK323, containing the recombinant plasmide pSK323 which provides for the oversynthesis of Eco RII enzymes. Data from gel filtration and Na-DS electrophoresis suggest that the restriction endonuclease Eco RII is a protein made up of two subunits, each with molecular weight of 44 000.
限制性内切酶Eco RII被分离并纯化至同质状态。分离过程涉及使用大肠杆菌菌株B834/pSK323，该菌株含有重组质粒pSK323，可实现Eco RII酶的过量合成。凝胶过滤和Na-DS电泳数据表明，限制性内切酶Eco RII是一种由两个亚基组成的蛋白质，每个亚基的分子量为44000。
