• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

极长链脂肪酸6基因产物的延长作用催化人体细胞中-13 : 0和-15 : 0奇数链饱和脂肪酸的延长。

The elongation of very long-chain fatty acid 6 gene product catalyses elongation of -13 : 0 and -15 : 0 odd-chain SFA in human cells.

作者信息

Wang Zhen, Wang Dong Hao, Goykhman Yuliya, Yan Yuanyuan, Lawrence Peter, Kothapalli Kumar S D, Brenna J Thomas

机构信息

Department of Food Science, Cornell University, Ithaca, NY 14853, USA.

Division of Nutritional Sciences, Cornell University, Ithaca, NY 14853, USA.

出版信息

Br J Nutr. 2019 Feb 14;121(3):241-248. doi: 10.1017/S0007114518003185. Epub 2019 Jan 3.

DOI:10.1017/S0007114518003185
PMID:30602402
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6610849/
Abstract

Normal odd-chain SFA (OCSFA), particularly tridecanoic acid (n-13 : 0), pentadecanoic acid (n-15 : 0) and heptadecanoic acid (n-17 : 0), are normal components of dairy products, beef and seafood. The ratio of n-15 : 0:n-17 : 0 in ruminant foods (dairy products and beef) is 2:1, while in seafood and human tissues it is 1:2, and their appearance in plasma is often used as a marker for ruminant fat intake. Human elongases encoded by elongation of very long-chain fatty acid (ELOVL)1, ELOVL3, ELOVL6 and ELOVL7 catalyse biosynthesis of the dominant even-chain SFA; however, there are no reports of elongase function on OCSFA. ELOVL transfected MCF7 cells were treated with n-13 : 0, n-15 : 0 or n-17 : 0 (80 µm) and products analysed. ELOVL6 catalysed elongation of n-13 : 0→n-15 : 0 and n-15 : 0→n-17 : 0; and ELOVL7 had modest activity toward n-15 : 0 (n-15 : 0→n-17 : 0). No elongation activity was detected for n-17 : 0→n-19 : 0. Our data expand ELOVL specificity to OCSFA, providing the first molecular evidence demonstrating ELOVL6 as the major elongase acting on OCSFA n-13 : 0 and n-15 : 0 fatty acids. Studies of food intake relying on OCSFA as a biomarker should consider endogenous human metabolism when relying on OCSFA ratios to indicate specific food intake.

摘要

正常的奇数链饱和脂肪酸（OCSFA），特别是十三烷酸（n-13 : 0）、十五烷酸（n-15 : 0）和十七烷酸（n-17 : 0），是乳制品、牛肉和海鲜的正常成分。反刍动物食物（乳制品和牛肉）中n-15 : 0与n-17 : 0的比例为2:1，而在海鲜和人体组织中为1:2，它们在血浆中的出现常被用作反刍动物脂肪摄入量的标志物。由超长链脂肪酸延长酶（ELOVL）1、ELOVL3、ELOVL6和ELOVL7编码的人类延长酶催化主要的偶数链饱和脂肪酸的生物合成；然而，尚无关于延长酶对OCSFA功能的报道。用n-13 : 0、n-15 : 0或n-17 : 0（80 µm）处理转染ELOVL的MCF7细胞，并分析产物。ELOVL6催化n-13 : 0→n-15 : 0和n-15 : 0→n-17 : 0的延长反应；ELOVL7对n-15 : 0（n-15 : 0→n-17 : 0）有适度活性。未检测到n-17 : 0→n-19 : 0的延长活性。我们的数据将ELOVL的特异性扩展到OCSFA，提供了首个分子证据证明ELOVL6是作用于OCSFA的n-13 : 0和n-15 : 0脂肪酸的主要延长酶。依靠OCSFA作为生物标志物进行食物摄入量研究时，在依靠OCSFA比例来指示特定食物摄入量时应考虑人体的内源性代谢。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c02/6610849/a5a9c14b1f73/nihms-1510010-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c02/6610849/54b50bd76be0/nihms-1510010-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c02/6610849/4cd2be45f264/nihms-1510010-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c02/6610849/49d5a86ead47/nihms-1510010-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c02/6610849/0602d7e32aa7/nihms-1510010-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c02/6610849/a5a9c14b1f73/nihms-1510010-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c02/6610849/54b50bd76be0/nihms-1510010-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c02/6610849/4cd2be45f264/nihms-1510010-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c02/6610849/49d5a86ead47/nihms-1510010-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c02/6610849/0602d7e32aa7/nihms-1510010-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c02/6610849/a5a9c14b1f73/nihms-1510010-f0005.jpg

相似文献

1
The elongation of very long-chain fatty acid 6 gene product catalyses elongation of -13 : 0 and -15 : 0 odd-chain SFA in human cells.极长链脂肪酸6基因产物的延长作用催化人体细胞中-13 : 0和-15 : 0奇数链饱和脂肪酸的延长。
Br J Nutr. 2019 Feb 14;121(3):241-248. doi: 10.1017/S0007114518003185. Epub 2019 Jan 3.
2
Identification of genes mediating branched chain fatty acid elongation.鉴定介导支链脂肪酸延伸的基因。
FEBS Lett. 2019 Jul;593(14):1807-1817. doi: 10.1002/1873-3468.13451. Epub 2019 May 30.
3
Odd chain fatty acid metabolism in mice after a high fat diet.高脂肪饮食后小鼠奇数链脂肪酸代谢。
Int J Biochem Cell Biol. 2022 Feb;143:106135. doi: 10.1016/j.biocel.2021.106135. Epub 2021 Dec 10.
4
Two Elongases, Elovl4 and Elovl6, Fulfill the Elongation Routes of the LC-PUFA Biosynthesis Pathway in the Orange Mud Crab ().两种延伸酶（Elovl4 和 Elovl6）在中华绒螯蟹（）中完成了 LC-PUFA 生物合成途径的延伸途径。
J Agric Food Chem. 2020 Apr 8;68(14):4116-4130. doi: 10.1021/acs.jafc.9b06692. Epub 2020 Mar 30.
5
5,5-Dimethyl-3-(5-methyl-3-oxo-2-phenyl-2,3-dihydro-1H-pyrazol-4-yl)-1-phenyl-3-(trifluoromethyl)-3,5,6,7-tetrahydro-1H-indole-2,4-dione, a potent inhibitor for mammalian elongase of long-chain fatty acids family 6: examination of its potential utility as a pharmacological tool.5,5-二甲基-3-(5-甲基-3-氧代-2-苯基-2,3-二氢-1H-吡唑-4-基)-1-苯基-3-(三氟甲基)-3,5,6,7-四氢-1H-吲哚-2,4-二酮，一种对哺乳动物长链脂肪酸家族6延长酶的强效抑制剂：对其作为药理学工具的潜在效用的研究。
J Pharmacol Exp Ther. 2009 Jul;330(1):249-56. doi: 10.1124/jpet.109.150854. Epub 2009 Apr 9.
6
Glioblastoma Multiforme Tumors in Women Have a Lower Expression of Fatty Acid Elongases , , , and than in Men.多形性胶质母细胞瘤在女性患者中的脂肪酸延长酶、、和的表达低于男性患者。
Brain Sci. 2022 Oct 6;12(10):1356. doi: 10.3390/brainsci12101356.
7
Fatty acid elongase 6 plays a role in the synthesis of long-chain fatty acids in goat mammary epithelial cells.脂肪酸延长酶 6 在山羊乳腺上皮细胞中长链脂肪酸的合成中发挥作用。
J Dairy Sci. 2017 Jun;100(6):4987-4995. doi: 10.3168/jds.2016-12159. Epub 2017 Apr 5.
8
Genome-wide association meta-analysis of circulating odd-numbered chain saturated fatty acids: Results from the CHARGE Consortium.全基因组关联荟萃分析循环奇数链饱和脂肪酸：CHARGE 联盟的研究结果。
PLoS One. 2018 May 8;13(5):e0196951. doi: 10.1371/journal.pone.0196951. eCollection 2018.
9
High-throughput assay for long chain fatty acyl-CoA elongase using homogeneous scintillation proximity format.使用均相闪烁邻近法对长链脂肪酰辅酶A延长酶进行高通量检测。
Assay Drug Dev Technol. 2009 Apr;7(2):124-32. doi: 10.1089/adt.2008.181.
10
Saturated fatty acids are not off the hook.饱和脂肪酸也不能免责。
Nutr Metab Cardiovasc Dis. 2015 Dec;25(12):1071-8. doi: 10.1016/j.numecd.2015.09.010. Epub 2015 Oct 9.

引用本文的文献

1
extract and chlorogenic acid attenuates high-fat-diet- induced prediabetes CTRPs-AdipoRs-AMPK/PPARα axes.提取物和绿原酸通过CTRPs - AdipoRs - AMPK/PPARα轴减轻高脂饮食诱导的糖尿病前期症状。
Front Nutr. 2022 Oct 14;9:1007679. doi: 10.3389/fnut.2022.1007679. eCollection 2022.
2
Simulated Night-Shift Schedule Disrupts the Plasma Lipidome and Reveals Early Markers of Cardiovascular Disease Risk.模拟夜班时间表扰乱血浆脂质组并揭示心血管疾病风险的早期标志物。
Nat Sci Sleep. 2022 May 21;14:981-994. doi: 10.2147/NSS.S363437. eCollection 2022.
3
Can individual fatty acids be used as functional biomarkers of dairy fat consumption in relation to cardiometabolic health? A narrative review.

本文引用的文献

1
Functional Characterization of Two Elongases of Very Long-Chain Fatty Acid from Tenebrio molitor L. (Coleoptera: Tenebrionidae).鞘翅目（Coleoptera：Tenebrionidae）家蝇（Tenebrio molitor L.）中两种超长链脂肪酸延长酶的功能特征。
Sci Rep. 2017 Sep 8;7(1):10990. doi: 10.1038/s41598-017-11134-y.
2
Molecular and functional characterisation of two elovl4 elongases involved in the biosynthesis of very long-chain (>C) polyunsaturated fatty acids in black seabream Acanthopagrus schlegelii.参与黑鲷（Acanthopagrus schlegelii）超长链（>C）多不饱和脂肪酸生物合成的两种elovl4延长酶的分子和功能表征
Comp Biochem Physiol B Biochem Mol Biol. 2017 Oct;212:41-50. doi: 10.1016/j.cbpb.2017.06.008. Epub 2017 Jun 29.
3
个体脂肪酸能否作为与心血管代谢健康有关的乳制品脂肪摄入的功能性生物标志物？ 一篇叙述性综述。
Br J Nutr. 2022 Dec 28;128(12):2373-2386. doi: 10.1017/S0007114522000289. Epub 2022 Jan 28.
4
Odd chain fatty acid metabolism in mice after a high fat diet.高脂肪饮食后小鼠奇数链脂肪酸代谢。
Int J Biochem Cell Biol. 2022 Feb;143:106135. doi: 10.1016/j.biocel.2021.106135. Epub 2021 Dec 10.
5
Plasma Docosahexaenoic Acid and Eicosapentaenoic Acid Concentrations Are Positively Associated with Brown Adipose Tissue Activity in Humans.血浆二十二碳六烯酸和二十碳五烯酸浓度与人体棕色脂肪组织活性呈正相关。
Metabolites. 2020 Sep 28;10(10):388. doi: 10.3390/metabo10100388.
6
Quantitative Phosphoproteomic Analysis Reveals the Regulatory Networks of on Lipid and Glucose Metabolism in Zebrafish.定量磷酸化蛋白质组学分析揭示了在斑马鱼脂质和糖代谢中的调控网络。
Int J Mol Sci. 2020 Apr 19;21(8):2860. doi: 10.3390/ijms21082860.
CONFIDENCE LIMITS ON PHYLOGENIES: AN APPROACH USING THE BOOTSTRAP.
系统发育树的置信区间：一种使用自展法的方法。
Evolution. 1985 Jul;39(4):783-791. doi: 10.1111/j.1558-5646.1985.tb00420.x.
4
Functional characterization and differential nutritional regulation of putative Elovl5 and Elovl4 elongases in large yellow croaker (Larimichthys crocea).大黄花鱼（Larimichthys crocea）中假定的 Elovl5 和 Elovl4 延长酶的功能特征和差异营养调控。
Sci Rep. 2017 May 23;7(1):2303. doi: 10.1038/s41598-017-02646-8.
5
Odd-chain fatty acids as a biomarker for dietary fiber intake: a novel pathway for endogenous production from propionate.奇数链脂肪酸作为膳食纤维摄入量的生物标志物：丙酸内源性产生的新途径。
Am J Clin Nutr. 2017 Jun;105(6):1544-1551. doi: 10.3945/ajcn.117.152702. Epub 2017 Apr 19.
6
Tissue expression profiles and transcriptional regulation of elongase of very long chain fatty acid 6 in bovine mammary epithelial cells.牛乳腺上皮细胞中极长链脂肪酸延长酶6的组织表达谱及转录调控
PLoS One. 2017 Apr 17;12(4):e0175777. doi: 10.1371/journal.pone.0175777. eCollection 2017.
7
Fatty acid elongase 6 plays a role in the synthesis of long-chain fatty acids in goat mammary epithelial cells.脂肪酸延长酶 6 在山羊乳腺上皮细胞中长链脂肪酸的合成中发挥作用。
J Dairy Sci. 2017 Jun;100(6):4987-4995. doi: 10.3168/jds.2016-12159. Epub 2017 Apr 5.
8
Odd Chain Fatty Acids; New Insights of the Relationship Between the Gut Microbiota, Dietary Intake, Biosynthesis and Glucose Intolerance.奇数链脂肪酸；肠道微生物群、饮食摄入、生物合成与葡萄糖不耐受关系的新见解。
Sci Rep. 2017 Mar 23;7:44845. doi: 10.1038/srep44845.
9
BCFA suppresses LPS induced IL-8 mRNA expression in human intestinal epithelial cells.丁酸梭菌发酵产物提取物抑制脂多糖诱导的人肠上皮细胞白细胞介素-8信使核糖核酸表达。
Prostaglandins Leukot Essent Fatty Acids. 2017 Jan;116:27-31. doi: 10.1016/j.plefa.2016.12.001. Epub 2016 Dec 5.
10
Milk fat biomarkers and cardiometabolic disease.乳脂肪生物标志物与心脏代谢疾病
Curr Opin Lipidol. 2017 Feb;28(1):46-51. doi: 10.1097/MOL.0000000000000381.