Bae Yun Ju, Cho Chi Heung, Lee Woo Jong, Huh Jeung Soo, Lim Jeong Ok
1Department of Biomedical Science, Graduate School, Kyungpook National University, Daegu, Korea.
Korea Institute of Industrial Technology, Biomedical Manufacturing Technology Center, Daegu, Korea.
Tissue Eng Regen Med. 2016 Feb 2;13(1):13-20. doi: 10.1007/s13770-015-0029-z. eCollection 2016 Feb.
Growth factors play multiple and critical roles in wound repair processes. Platelet-derived growth factor (PDGF) is a potent growth factor that is particularly important in the early inflammatory phase of wound healing. In order to extend the half-life of PDGF, polymeric encapsulation is used. In the current study, Poly (lactic-co-glycolic acid) (PLGA) microspheres containing recombinant human (rh) PDGF-BB were prepared to prolong the effectiveness of this growth factor. PLGA microspheres were optimized using a modified w/o/w-double-emulsion/solvent evaporation method by changing the processing conditions of stirring speed and emulsifier (polyvinyl alcohol) concentration. Microspheres prepared using the optimized method released rhPDGF-BB for up to three weeks. An migration assay showed a significant decrease in the wound area in cells treated with rhPDGF-BB microspheres compared to control cells. These findings demonstrate the potential of rhPDGF-BB encapsulated in microspheres to enhance wound healing.
生长因子在伤口修复过程中发挥着多重关键作用。血小板衍生生长因子（PDGF）是一种强效生长因子，在伤口愈合的早期炎症阶段尤为重要。为了延长PDGF的半衰期，采用了聚合物封装技术。在本研究中，制备了含有重组人（rh）PDGF - BB的聚（乳酸 - 乙醇酸）（PLGA）微球，以延长这种生长因子的有效性。通过改变搅拌速度和乳化剂（聚乙烯醇）浓度的加工条件，使用改良的水包油包水双乳液/溶剂蒸发法对PLGA微球进行了优化。采用优化方法制备的微球可释放rhPDGF - BB长达三周。迁移试验表明，与对照细胞相比，用rhPDGF - BB微球处理的细胞伤口面积显著减小。这些发现证明了微球封装的rhPDGF - BB在促进伤口愈合方面的潜力。
