Park Jung-Hyun, Kim Ki-Joo, Rhie Jong Won, Oh Il-Hoan
1Department of Medical Life Science, Catholic High-Performance Cell Therapy Center, College of Medicine, The Catholic University of Korea, Seoul, Korea.
2Department of Plastic Surgery, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Tissue Eng Regen Med. 2016 Feb 2;13(1):39-46. doi: 10.1007/s13770-015-0027-1. eCollection 2016 Feb.
Mesenchymal stromal cells (MSCs) established by adherence culture have been widely utilized for various cell therapeutic trials, but potential heterogeneity that can be caused by preparation methods are poorly characterized. In this study, we show that at least two distinct subsets of MSCs with different adherence to plastic surface exist in human adipose tissue-derived stromal vascular fraction (SVF); while 69% of total colony forming units in SVF adhere to the surface before 3 hrs of plating, 13-17% of colonogenic cells adhered to the surface at later period of 15 hr to 1 week after plating. Of note, the late adherent MSCs exhibited higher self-renewal of colony forming cells and higher proliferating potential with comparable level of osteogenic or adipogenic differentiation potential to the early adherence subsets. Moreover, late adherent cells exhibited distinct pattern of paracrine secretome including higher level secretion of cytokines than the early adherent subsets. Taken together, these results suggest the possibility that distinct adherence properties of MSCs can be another parameter of clonal heterogeneity in the subpopulations of adipose tissue MSCs and that it can be an important factor for optimization of MSC preparation for cell therapeutic trials.
通过贴壁培养建立的间充质基质细胞（MSCs）已被广泛用于各种细胞治疗试验，但制备方法可能导致的潜在异质性却鲜有描述。在本研究中，我们发现人脂肪组织来源的基质血管成分（SVF）中存在至少两个不同的MSCs亚群，它们对塑料表面的粘附性不同；虽然SVF中69%的总集落形成单位在接种后3小时内粘附于表面，但13 - 17%的成集落细胞在接种后15小时至1周的后期才粘附于表面。值得注意的是，晚期粘附的MSCs表现出更高的集落形成细胞自我更新能力和更高的增殖潜力，其成骨或成脂分化潜力与早期粘附亚群相当。此外，晚期粘附细胞表现出独特的旁分泌分泌组模式，包括比早期粘附亚群更高水平的细胞因子分泌。综上所述，这些结果表明，MSCs不同的粘附特性可能是脂肪组织MSCs亚群中克隆异质性的另一个参数，并且它可能是优化细胞治疗试验中MSCs制备的一个重要因素。
