Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, Minnesota, 55905.
Department of Orthopedic Surgery, Mayo Clinic, Rochester, Minnesota, 55905.
J Biomed Mater Res A. 2018 May;106(5):1247-1257. doi: 10.1002/jbm.a.36322. Epub 2018 Jan 23.
Graphene oxide (GO) is an attractive material that can be utilized to enhance the modulus and conductivities of substrates and hydrogels. To covalently cross-link graphene oxide sheets into hydrogels, abundant cross-linkable double bonds were introduced to synthesize the graphene-oxide-tris-acrylate sheet (GO-TrisA). Polyacrylamide (PAM) nanocomposite hydrogels were then fabricated with inherent covalently and permanently cross-linked GO-TrisA sheets. Results showed that the covalently cross-linked GO-TrisA/PAM nanocomposite hydrogel had enhanced mechanical strength, thermo stability compared with GO/PAM hydrogel maintained mainly by hydrogen bonding between PAM chains and GO sheets. In vitro cell study showed that the covalently cross-linked rGO-TrisA/PAM nanocomposite hydrogel had excellent cytocompatibility after in situ reduction. These results suggest that rGO-TrisA/PAM nanocomposite hydrogel holds great potential for tissue engineering applications. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1247-1257, 2018.
氧化石墨烯（GO）是一种很有吸引力的材料，可以用来提高基质和水凝胶的模量和电导率。为了将氧化石墨烯片共价交联成水凝胶，我们引入了丰富的可交联双键来合成氧化石墨烯-三丙烯酸酯片（GO-TrisA）。然后用固有共价和永久交联的 GO-TrisA 片制备聚丙烯酰胺（PAM）纳米复合水凝胶。结果表明，与主要通过 PAM 链和 GO 片之间氢键结合保持的 GO/PAM 水凝胶相比，共价交联的 GO-TrisA/PAM 纳米复合水凝胶具有更高的机械强度和热稳定性。体外细胞研究表明，原位还原后，共价交联的 rGO-TrisA/PAM 纳米复合水凝胶具有优异的细胞相容性。这些结果表明，rGO-TrisA/PAM 纳米复合水凝胶在组织工程应用中具有很大的潜力。© 2018 年 Wiley 期刊，生物医学材料研究杂志 A 部分：106A：1247-1257，2018 年。
