Kim Sook Kyoung, Cho Tae Hyung, Han Jeong Joon, Kim In Sook, Park Yongdoo, Hwang Soon Jung
1Department of Biomedical Engineering, College of Medicine, Korea University, Seoul, Korea.
2Department of Oral and Maxillofacial Surgery, Seoul National University Dental Hospital, School of Dentistry, Seoul National University, Seoul, Korea.
Tissue Eng Regen Med. 2016 Apr 5;13(2):171-181. doi: 10.1007/s13770-015-0046-y. eCollection 2016 Apr.
The effect of vascular endothelial growth factor (VEGF) combined with bone morphogenetic protein-2 (BMP-2) for bone regeneration is still controversial as to whether or not VEGF has a synergistic or additive effect. This study attempted to evaluate the synergistic effect of VEGF and BMP-2 compared to BMP-2 alone for maxillary alveolar bone regeneration using collagen sponge/hydrogel complex sheets in a canine model. After mixing BMP-2 and VEGF with a hyaluronic acid-based hydrogel (HAH), the collagen sponge/hydrogel complex was transplanted into maxillary alveolar bone defects (n=14) after the extraction of canine upper first molars on both sides. Bone regeneration was evaluated in three groups (control group without growth factors, experimental groups I and II with BMP-2 alone and BMP-2 and VEGF, respectively) using micro-computed tomography and histological staining. The total amount of new bone formations and bone mineral density were significantly higher in the group with BMP-2 only and the group with BMP-2 combined with VEGF than it in the control group. The area with positive staining of von Willebrand factor bone defect was significantly greater in the group with BMP-2 only and with dual growth factors than the control. BMP-2 released from the HAH promoted new bone formation. However, the combination of BMP-2 and VEGF did not show a synergistic or additive effect on bone regeneration at canine maxillary alveolar bone defects.
血管内皮生长因子（VEGF）与骨形态发生蛋白-2（BMP-2）联合用于骨再生时，VEGF是否具有协同或相加作用仍存在争议。本研究试图在犬模型中，使用胶原海绵/水凝胶复合片，评估VEGF和BMP-2联合与单独使用BMP-2相比，对上颌牙槽骨再生的协同作用。将BMP-2和VEGF与基于透明质酸的水凝胶（HAH）混合后，在拔除两侧犬上颌第一磨牙后，将胶原海绵/水凝胶复合物植入上颌牙槽骨缺损处（n = 14）。使用微型计算机断层扫描和组织学染色，在三组（无生长因子的对照组、单独使用BMP-2的实验组I和同时使用BMP-2和VEGF的实验组II）中评估骨再生情况。仅使用BMP-2的组和BMP-2与VEGF联合的组中，新骨形成总量和骨矿物质密度均显著高于对照组。仅使用BMP-2的组和使用双生长因子的组中，血管性血友病因子骨缺损阳性染色面积显著大于对照组。从HAH释放的BMP-2促进了新骨形成。然而，在犬上颌牙槽骨缺损处，BMP-2与VEGF联合对骨再生未显示出协同或相加作用。
