Ministry Education Key Laboratory for Oral Biomedical Engineering, School of Stomatology, Wuhan University, Wuhan, China.
Clin Oral Implants Res. 2012 Apr;23(4):467-73. doi: 10.1111/j.1600-0501.2011.02164.x. Epub 2011 Mar 28.
To evaluate the synergistic effect of bone morphogenetic protein 2 (BMP-2) and vascular endothelial growth factor (VEGF) on the repair of bone defects around dental implants.
Five groups of scaffold were fabricated by a freeze-drying method, including pure chitosan/collagen scaffold; scaffold loaded with adenoviruses expressing BMP-2, adenoviruses expressing VEGF, both adenoviruses expressing BMP-2 and VEGF, VEGF protein and adenovirus expressing BMP-2. In vitro studies examined whether bone marrow stromal cells were responsive to these scaffolds over time. Bone formation capacity, bone-to-implant contact, as well as removal torque values were investigated in vivo. Differences between the various groups were statistically analyzed using the one-way analysis of variance test.
The in vitro study revealed a burst and rapid release of VEGF with a sustained high-level expression of BMP-2 in scaffold combined with VEGF protein and adenoviruses expressing BMP-2. Histomorphometry demonstrated that scaffolds expressing BMP-2 enhanced more bone formation compared with other groups; VEGF alone is insufficient to promote bone formation. New bone formation in the bone defects around dental implants, bone-to-implant contact and mean peak removal torque showed statistically significant difference for the adenoviral vector encoding human bone morphogenetic protein 2 (Ad-BMP-2) and VEGF protein and adenovirus expressing BMP-2 groups. Furthermore, scaffold combined with VEGF protein and Ad-BMP-2 represented the best outcomes in this model.
A combination of BMP-2 gene and VEGF protein could have a synergistic effect in promoting bone healing.
评估骨形态发生蛋白 2（BMP-2）和血管内皮生长因子（VEGF）联合对种植体周围骨缺损修复的协同作用。
采用冷冻干燥法制备 5 组支架，包括纯壳聚糖/胶原支架；负载表达 BMP-2 的腺病毒、表达 VEGF 的腺病毒、同时负载表达 BMP-2 和 VEGF 的腺病毒、VEGF 蛋白和表达 BMP-2 的腺病毒的支架。体外研究检测骨髓基质细胞对这些支架随时间的反应。体内研究检测骨形成能力、骨-种植体接触以及去除扭矩值。采用单因素方差分析对各组间的差异进行统计学分析。
体外研究显示，VEGF 呈爆发式快速释放，与 VEGF 蛋白和表达 BMP-2 的腺病毒结合的支架中 BMP-2 呈持续高水平表达。组织形态计量学显示，与其他组相比，表达 BMP-2 的支架促进了更多的骨形成；单独使用 VEGF 不足以促进骨形成。种植体周围骨缺损中，新骨形成、骨-种植体接触和平均峰值去除扭矩在携带人骨形态发生蛋白 2（Ad-BMP-2）的腺病毒载体和 VEGF 蛋白和表达 BMP-2 的组之间存在统计学差异。此外，支架与 VEGF 蛋白和 Ad-BMP-2 联合使用在该模型中效果最佳。
BMP-2 基因和 VEGF 蛋白的联合使用可能具有协同促进骨愈合的作用。
