Lee Jaeyeon, Lee Se-Hwan, Lee Bu-Kyu, Park Sang-Hyug, Cho Young-Sam, Park Yongdoo
1Department of Biomedical Engineering, College of Medicine, Korea University, 73 Inchon-ro, Seongbuk-gu, Seoul, 02841 Republic of Korea.
2Department of Mechanical Design Engineering, College of Engineering, Wonkwang University, 460 Iksandae-ro, Iksan, Jeonbuk, 54538 Republic of Korea.
Tissue Eng Regen Med. 2018 Jul 10;15(4):403-413. doi: 10.1007/s13770-018-0130-1. eCollection 2018 Aug.
The fabrication of microchannels in hydrogel can facilitate the perfusion of nutrients and oxygen, which leads to guidance cues for vasculogenesis. Microchannel patterning in biomimetic hydrogels is a challenging issue for tissue regeneration because of the inherent low formability of hydrogels in a complex configuration. We fabricated microchannels using wire network molding and immobilized the angiogenic factors in the hydrogel and evaluated the vasculogenesis and .
Microchannels were fabricated in a hyaluronic acid-based biomimetic hydrogel by using "wire network molding" technology. Substance P was immobilized in acrylated hyaluronic acid for angiogenic cues using Michael type addition reaction. and angiogenic activities of hydrogel with microchannels were evaluated.
cell culture experiment shows that cell viability in two experimental biomimetic hydrogels (with microchannels and microchannels + SP) was higher than that of a biomimetic hydrogel without microchannels (bulk group). Evaluation on differentiation of human mesenchymal stem cells (hMSCs) in biomimetic hydrogels with fabricated microchannels shows that the differentiation of hMSC into endothelial cells was significantly increased compared with that of the bulk group. angiogenesis analysis shows that thin blood vessels of approximately 25-30 μm in diameter were observed in the microchannel group and microchannel + SP group, whereas not seen in the bulk group.
The strategy of fabricating microchannels in a biomimetic hydrogel and simultaneously providing a chemical cue for angiogenesis is a promising formula for large-scale tissue regeneration.
在水凝胶中制造微通道可促进营养物质和氧气的灌注，从而为血管生成提供引导线索。由于水凝胶在复杂构型中固有的低成型性，在仿生水凝胶中进行微通道图案化是组织再生中的一个具有挑战性的问题。我们使用金属丝网成型技术制造了微通道，并将血管生成因子固定在水凝胶中，然后评估了血管生成情况。
通过“金属丝网成型”技术在基于透明质酸的仿生水凝胶中制造微通道。使用迈克尔型加成反应将P物质固定在丙烯酸化透明质酸中以提供血管生成线索。对具有微通道的水凝胶的血管生成活性进行了评估。
细胞培养实验表明，两种实验性仿生水凝胶（有微通道和微通道+P物质）中的细胞活力高于无微通道的仿生水凝胶（块状组）。对具有制造微通道的仿生水凝胶中人间充质干细胞（hMSCs）分化的评估表明，与块状组相比，hMSC向内皮细胞的分化显著增加。血管生成分析表明，在微通道组和微通道+P物质组中观察到直径约25-30μm的细血管，而在块状组中未观察到。
在仿生水凝胶中制造微通道并同时为血管生成提供化学线索的策略是大规模组织再生的一个有前景的方案。
