College of Pharmacy, Keimyung University, 1095 Dalgubeol-daero, Dalseo-Gu, Daegu, 42601, Republic of Korea.
Komipharm International Co., Ltd., 17 Gyeongje-ro, Siheung-si, Gyonggi-do, 15094, Republic of Korea.
AAPS PharmSciTech. 2019 Jan 2;20(1):31. doi: 10.1208/s12249-018-1245-3.
We prepared mineral oil-based emulsion adjuvants by employing simple self-emulsifying drug delivery system (SEDDS). Mineral oil emulsions (3%, 5%, and 7%) were prepared using deionized water and C-971P NF and C-940 grade carbomer solutions with concentrations 0.01% (w/v) and 0.02% (w/v). In total, 15 emulsions were prepared and mixed with a solution containing inactivated Mycoplasma hyopneumoniae (J101 strain) antigen and porcine circovirus type 2 antigen to prepare vaccines. Droplet sizes in the submicron range and zeta potential values between - 40 and 0 mV were maintained by most emulsion adjuvants for a period of 6 months. Emulsion adjuvants were regarded safe, and their M. hyopneumoniae-specific IgG, IgG1, and IgG2a titers were either better or comparable to those of aluminum gel.
我们采用简单的自乳化药物传递系统（SEDDS）来制备矿物油基乳剂佐剂。使用去离子水和 C-971P NF 和 C-940 级卡波姆溶液（浓度为 0.01%（w/v）和 0.02%（w/v））制备 3%、5%和 7%的矿物油乳液。总共制备了 15 种乳剂，并与含有灭活的支原体肺炎（J101 株）抗原和猪圆环病毒 2 型抗原的溶液混合制备疫苗。大多数乳剂佐剂在 6 个月的时间内保持亚微米范围内的液滴大小和-40 至 0 mV 之间的 ζ 电位值。乳剂佐剂被认为是安全的，它们针对支原体肺炎的特异性 IgG、IgG1 和 IgG2a 滴度要么更好，要么与铝凝胶相当。
