Graduate Institute of Pharmacy, National Defense Medical Center, Taipei, Taiwan, Republic of China.
School of Pharmacy, National Defense Medical Center, R9304, No.161, Sec. 6, Min-Chuan E. Rd., Neihu District, Taipei 114, Taiwan, Republic of China.
Pharm Res. 2019 Jan 2;36(2):32. doi: 10.1007/s11095-018-2550-y.
Various blood collection methods were developed and used in the pharmacokinetic evaluation of drugs. However, the influence of different blood sampling methods on plasma drug concentrations has not been clarified. In the present study, we aimed to determine whether the plasma concentration of a target drug changes based on the collection site and elucidate the mechanism responsible for this change.
We compared three blood sampling methods commonly used in small animals. Eight clinical drugs were selected and administered to rats simultaneously via intracardiac injection or oral gavage. Blood samples were collected from different sites at the same individual, and pharmacokinetic properties of the drugs were then evaluated.
Study results showed that the maximum plasma concentration or area under the curve of three study drugs was significantly higher in rats when blood was sampled from the carotid artery than when it was sampled from the caudal vein or by tail snip.
Pharmacokinetics of certain drugs may differ based on the blood sampling site. The acid-base properties of drugs may influence pharmacokinetic evaluation. The rate and extent of drug distribution may also cause such differences and have significant effects on plasma drug levels.
开发并使用了各种血液采集方法来进行药物的药代动力学评估。然而，不同的血液采样方法对血浆药物浓度的影响尚未阐明。在本研究中，我们旨在确定基于采集部位，目标药物的血浆浓度是否会发生变化，并阐明导致这种变化的机制。
我们比较了三种常用于小动物的血液采样方法。同时通过心内注射或口服灌胃给予大鼠 8 种临床药物。从同一个体的不同部位采集血样，并评估药物的药代动力学特性。
研究结果表明，与从尾静脉或尾尖采血相比，从颈动脉采血时，三种研究药物中的三种药物的最大血浆浓度或曲线下面积在大鼠中显著升高。
某些药物的药代动力学可能会因采血部位的不同而有所差异。药物的酸碱性质可能会影响药代动力学评估。药物的分布速率和程度也可能导致这种差异，并对血浆药物水平产生重大影响。
