Jafari Seyyed Mehdi, Nazri Ali, Shabani Mahdi, Balajam Narges Zargar, Aghaei Mahmoud
Metabolic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, I.R. Iran.
Department of Biochemistry and Biophysics, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, I.R. Iran.
Res Pharm Sci. 2018 Dec;13(6):557-565. doi: 10.4103/1735-5362.245967.
Galectin-9 (Gal-9), a member of animal lectins' family, is implicated in the induction of apoptosis in various cancer cells. Here, we evaluated the anti-tumor effect of Gal-9 in OVCAR-3 ovarian cancer cells. The effect of the Gal-9 on cell viability was evaluated using MTT assays. Apoptosis was assessed using Annexin-V staining. The assessment of mitochondrial membrane potential (ΔΨm) was performed using a JC-1 probe. The activity of caspase-3 and caspase-6 were evaluated with colorimetric assay. The production of reactive oxygen species (ROS) was applied by fluorescent probe. The expression levels of Bax and Bcl-2 were assessed using western blotting. The result showed that Gal-9 inhibits cell viability. Flow cytometry analysis showed that Gal-9 induces apoptosis in ovarian cancer cells. Moreover, Gal-9 decreased ΔΨm and increased the generation of ROS and caspase-3 and caspase-6 activities in ovarian cancer cells. Moreover, Gal-9 induced expression of Bax as well as inhibited expression of Bcl-2. In conclusion, our results indicated that Gal-9 induced apoptosis in ovarian cancer cells through mitochondrial pathway.
半乳糖凝集素-9（Gal-9）是动物凝集素家族的成员之一，与多种癌细胞的凋亡诱导有关。在此，我们评估了Gal-9对OVCAR-3卵巢癌细胞的抗肿瘤作用。使用MTT法评估Gal-9对细胞活力的影响。使用膜联蛋白V染色评估细胞凋亡。使用JC-1探针评估线粒体膜电位（ΔΨm）。用比色法评估半胱天冬酶-3和半胱天冬酶-6的活性。通过荧光探针检测活性氧（ROS）的产生。使用蛋白质印迹法评估Bax和Bcl-2的表达水平。结果表明，Gal-9抑制细胞活力。流式细胞术分析表明，Gal-9诱导卵巢癌细胞凋亡。此外，Gal-9降低了卵巢癌细胞的ΔΨm，并增加了ROS的产生以及半胱天冬酶-3和半胱天冬酶-6的活性。此外，Gal-9诱导Bax的表达并抑制Bcl-2的表达。总之，我们的结果表明，Gal-9通过线粒体途径诱导卵巢癌细胞凋亡。
