Peptide Institute, Inc., Ibaraki, Osaka 567-0085, Japan.
Org Biomol Chem. 2019 Jan 23;17(4):776-779. doi: 10.1039/c8ob02766h.
The first chromogenic substrate for Aspergillus oryzae acid protease, 'HAP-01', was successfully developed after evaluating the substrate specificity of the enzyme. Furthermore, with HAP-01, digestion-triggered chromophore release was employed as a novel chromogenic technique.
经过评估该酶的底物特异性,成功开发出米曲霉酸性蛋白酶的第一种生色底物 'HAP-01'。此外,利用 HAP-01,我们采用了消化触发生色团释放的新型生色技术。