School of Pharmaceutical Sciences, Jaipur National University, Jaipur, India.
Bioactive Natural Product Laboratory, School of Pharmaceutical Education and Research, New Delhi, India.
Phytochem Anal. 2019 May;30(3):332-345. doi: 10.1002/pca.2816. Epub 2019 Jan 4.
The absence of microbial growth and resistance to oxidative deterioration in fruits of Musa × paradisiaca L. (bananas) is an indication of the presence of antimicrobial and antioxidant metabolites.
In order to investigate the secondary metabolomic spectrum as well as the active antimicrobial and antioxidants present in essential oils (EOs) from fruits of different geographical areas of M. × paradisiaca, gas chromatography-mass spectroscopy (GC-MS) principal component data correlation analysis is complemented with antimicrobial assays and phytochemical and bioautographic antioxidant fingerprints with thin layer chromatography (TLC).
An EO was obtained by steam distillation and subjected to GC-MS and TLC for metabolomic profiling from fruit pulp. The antimicrobial potential was tested in both Escherichia coli as a gram negative and Bacillus subtilis as a gram positive microbe. Potential antioxidant metabolites were identified through TLC-bioautography and GC-MS analysis of active zones.
A maximum of 0.56% v/w EO was isolated from fruit pulps of M. × paradisiaca. Minimum inhibitory concentrations (MICs) against B. subtillis and E. coli were 0.25 and 0.35 μg/mL, respectively. Thus, 56 metabolites were identified through GC-MS. The major abundant antimicrobial metabolites found in EOs are α-thujene, γ-terpinene, α- and β-pinene, sabinene, β-myrcene, limonene, α-capaene, caryophyllene and (Z,E)-α farnesene. Aceteugenol, palmitic acid, stearic acid, palmitin, and stearin were identified as antioxidant metabolites. Principal component analysis of metabolite data reveals correlations and a clear separation based on metabolites obtained from various areas.
The data generated using metabolic profiling and cluster analysis helped to identify antimicrobial and antioxidant compounds in M. × paradisiaca.
在 Musa × paradisiaca L.（香蕉）果实中没有微生物生长和对氧化恶化的抗性，这表明存在抗菌和抗氧化代谢物。
为了研究不同地理区域 M. × paradisiaca 果实精油（EOs）中的次生代谢物谱以及存在的抗菌和抗氧化活性物质，气相色谱-质谱联用（GC-MS）主成分数据分析与抗菌测定以及植物化学和薄层色谱（TLC）生物自显影抗氧化指纹图谱相补充。
通过蒸汽蒸馏从果肉中获得 EO，并进行 GC-MS 和 TLC 代谢组学分析。在革兰氏阴性大肠杆菌和革兰氏阳性芽孢杆菌中测试了抗菌潜力。通过 TLC-生物自显影和 GC-MS 分析活性区域来鉴定潜在的抗氧化代谢物。
从 M. × paradisiaca 果肉中最多可分离出 0.56%（v/w）的 EO。对枯草芽孢杆菌和大肠杆菌的最小抑菌浓度（MIC）分别为 0.25 和 0.35μg/mL。因此，通过 GC-MS 鉴定了 56 种代谢物。在 EOs 中发现的主要丰富的抗菌代谢物是α-蒎烯、γ-萜品烯、α-和β-蒎烯、柠檬烯、β-月桂烯、柠檬烯、α-石竹烯、石竹烯和（Z，E）-α金合欢烯。鉴定出乙酰丁香酚、棕榈酸、硬脂酸、棕榈酸酯和硬脂酸酯为抗氧化代谢物。代谢物数据的主成分分析根据从不同地区获得的代谢物揭示了相关性和明显的分离。
使用代谢物谱分析和聚类分析生成的数据有助于鉴定 M. × paradisiaca 中的抗菌和抗氧化化合物。
