Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, 730070 Lanzhou, China.
Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, 730070 Lanzhou, China.
Talanta. 2019 Mar 1;194:493-500. doi: 10.1016/j.talanta.2018.09.111. Epub 2018 Oct 22.
This paper developed a sensitive method for detecting hydroquinone by combining the unique optical properties of quantum dots and the specificity of enzymatic reactions. The interesting results shown that the fluorescence of graphite phase carbon nitride quantum dot (g-CNQDs) was quenched directly by benzoquinone or indirectly by hydroquinone (HQ), and the possible quenching mechanism was proposed. Quinone might generate by catalyzing the oxidation of HQ with horseradish peroxidase (HRP) in the presence of hydrogen peroxide. The intermediate quinone could effectively quench the fluorescence of g-CNQDs quantum dots. Therefore, a novel fluorescence probe based on g-CNQDs Quantum Dots was successfully used to detect HQ by strongly quenching the fluorescence of g-CNQDs Quantum Dots which mediated by horseradish peroxidase enzymes. The detection limit was as low as 0.04 μM (S/N = 3), and the linear range was 0.5-11.6 μM. This kind of quantum dot-enzyme system has the advantages of simple operation, no modification of quantum dots, low cost, high sensitivity and short detection time.
本文开发了一种灵敏的检测对苯二酚的方法，将量子点的独特光学性质和酶反应的特异性结合在一起。有趣的结果表明，石墨相氮化碳量子点（g-CNQDs）的荧光被苯醌直接猝灭，或通过间苯二酚（HQ）间接猝灭，提出了可能的猝灭机制。在存在过氧化氢的情况下，辣根过氧化物酶（HRP）催化 HQ 的氧化，可能会生成醌。中间醌可以有效地猝灭 g-CNQDs 量子点的荧光。因此，成功地利用 g-CNQDs 量子点通过辣根过氧化物酶酶介导的强烈猝灭 g-CNQDs 量子点的荧光，基于 g-CNQDs 量子点的新型荧光探针被用来检测 HQ。检测限低至 0.04μM（S/N=3），线性范围为 0.5-11.6μM。这种量子点-酶体系具有操作简单、无需量子点修饰、成本低、灵敏度高、检测时间短等优点。
