REQUIMTE/LAQV, Instituto Superior de Engenharia do Porto (ISEP), Politécnico do Porto, Rua Dr. António Bernardino de Almeida 431, 4200-072 Porto, Portugal.
CIQUP, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto (FCUP), Rua do Campo Alegre, s/n, 4169-007 Porto, Portugal.
Talanta. 2019 Mar 1;194:689-696. doi: 10.1016/j.talanta.2018.10.090. Epub 2018 Oct 29.
Recent studies have shown that besides the well-known T (triiodothyronine) and T (thyroxine) there might be other important thyroid hormones, in particular TAM (thyronamine) and TAM (3-iodothyronamine). The absence of a large number of studies showing their precise importance might be explained by the limited number of analytical methodologies available. This work aims to show an electroanalytical alternative making use of electropolymerized molecularly imprinted polymer (MIPs). The MIPs' polymerization is performed on the surface of screen-printed carbon electrodes (SPCEs), using 4-aminobenzoic acid (4-ABA) as the building and functional monomer and the analyte TAM as the template. The step-by-step construction of the SPCE-MIP sensor was studied by cyclic voltammetry (CV) and by electrochemical impedance spectroscopy (EIS). After optimization, by means of square-wave voltammetry, the SPCE-MIP showed suitable selectivity (in comparison with other thyroid hormones and catechol amines), repeatability (intra-day of 3.9%), a linear range up to 10 μmol L (0.23 × 10 μg dL) with an r of 0.998 and a limit of detection (LOD) and quantification (LOQ) of 0.081 and 0.27 μmol L (1.9 and 6.2 μg dL), respectively.
最近的研究表明，除了众所周知的 T（三碘甲状腺原氨酸）和 T（甲状腺素）之外，可能还有其他重要的甲状腺激素，特别是 TAM（甲状腺胺）和 TAM（3-碘甲状腺原氨酸）。缺乏大量研究表明它们的确切重要性，可能是由于可用的分析方法数量有限。本工作旨在展示一种利用电化学聚合分子印迹聚合物（MIPs）的电分析替代方法。MIPs 的聚合是在丝网印刷碳电极（SPCE）的表面上进行的，使用 4-氨基苯甲酸（4-ABA）作为构建和功能单体，分析物 TAM 作为模板。通过循环伏安法（CV）和电化学阻抗谱（EIS）研究了 SPCE-MIP 传感器的逐步构建。通过方波伏安法进行优化后，SPCE-MIP 显示出合适的选择性（与其他甲状腺激素和儿茶酚胺相比）、重复性（日内 3.9%）、线性范围高达 10 μmol L（0.23×10 μg dL），r 为 0.998，检测限（LOD）和定量限（LOQ）分别为 0.081 和 0.27 μmol L（1.9 和 6.2 μg dL）。
