长链非编码RNA RP11-397A15.4在胃癌中的表达增加及其临床意义

Increased Expression of Lncrna RP11-397A15.4 in Gastric Cancer and Its Clinical Significance.

作者信息

Chen Hui, Xu Zhiying, Liu Xiangfeng, Gao Yi, Wang Juan, Qian Peng, Yang Bin

机构信息

Department of Gastroenterology, People's Hospital of Taizhou, Taizhou, Jiangsu, China.

Department of General Surgery, 2 Xiangya Hospital, Central South University, Changsha, Hunan, China.

出版信息

Ann Clin Lab Sci. 2018 Nov;48(6):707-711.

PMID:30610039

Abstract

BACKGROUND

Long noncoding RNAs (lncRNAs) have been demonstrated to have potential diagnostic and prognostic values for many types of cancers. However, their clinical value in patients with gastric cancer (GC) remains unknown.

METHODS

The expression levels of lncRNA RP11-397A15.4 in 68 GC tissues and their matched adjacent non-tumor tissues from GC patients and in 24 normal mucosa or those with minimal gastritis (healthy controls) were determined by qRT-PCR. The expression levels of lncRNA RP11-397A15.4 in human GC cell lines and a normal gastric epithelium cell line were also detected by qRT-PCR. The potential associations between lncRNA RP11-397A15.4 expression level in GC tissues and the clinicopathological features of GC patients were analyzed. Moreover, a receiver operating characteristic (ROC) curve was constructed for differentiating patients with gastric cancer from patients with benign gastric diseases. In addition, the correlation between lncRNA RP11-397A15.4 and overall survival (OS) was analyzed.

RESULTS

The expression levels of lncRNA RP11-397A15.4 were significantly reduced in GC tissues and cell lines. Increased expression of lncRNA RP11-397A15.4 was associated with tumor differentiation, clinical stage, and distant metastasis. Furthermore, the area under the ROC curve (AUC) was up to 0.9726 (95%CI=0.9492-0.9961, <0.0001). The correlation between lncRNA RP11-397A15.4 expression and overall survival (OS) was statistically significant.

CONCLUSION

The present study demonstrated that lncRNA RP11-397A15.4 was highly expressed in GC tissues and may serve as a potential diagnostic and prognostic biomarker for GC.

摘要

背景

长链非编码RNA（lncRNAs）已被证明对多种癌症具有潜在的诊断和预后价值。然而，它们在胃癌（GC）患者中的临床价值仍不清楚。

方法

通过qRT-PCR测定68例GC患者的GC组织及其匹配的相邻非肿瘤组织以及24例正常黏膜或轻度胃炎患者（健康对照）中lncRNA RP11-397A15.4的表达水平。还通过qRT-PCR检测了人GC细胞系和正常胃上皮细胞系中lncRNA RP11-397A15.4的表达水平。分析了GC组织中lncRNA RP11-397A15.4表达水平与GC患者临床病理特征之间的潜在关联。此外，构建了用于区分胃癌患者和良性胃病患者的受试者工作特征（ROC）曲线。另外，分析了lncRNA RP11-397A15.4与总生存期（OS）之间的相关性。

结果

lncRNA RP11-397A15.4在GC组织和细胞系中的表达水平显著降低。lncRNA RP11-397A15.4表达增加与肿瘤分化、临床分期和远处转移相关。此外，ROC曲线下面积（AUC）高达0.9726（95%CI = 0.9492 - 0.9961，P < 0.0001）。lncRNA RP11-397A15.4表达与总生存期（OS）之间的相关性具有统计学意义。