Yu Liqiang, Li Jie, Chen Yujue, Jiang Juean, Fang Qi, Jiang Jianhua, Wang Dapeng, Liu Meirong
Department of Neurology and VIP Ward, The First Affiliated Hospital of Soochow University, Suzhou, China
Department of Neurology and VIP Ward, The First Affiliated Hospital of Soochow University, Suzhou, China.
Ann Clin Lab Sci. 2018 Nov;48(6):764-769.
microRNAs (miRNAs) have been identified as biomarkers for various diseases. However, the significance of circulating miRNAs for the diagnosis of idiopathic inflammatory myopathies (IIM) is still unknown. In this study, we aim to investigate the significance of miRNAs as potential biomarkers for predicting the patients with IIM and interstitial lung disease (ILD) METHODS: Total RNA was isolated from plasma of 43 IIM patients and 43 healthy people. The expression of miRNAs was analyzed by miRNA microarray and validated by qRT-PCR RESULTS: Microarray shows more differentially expressed circulating miRNAs found in IIM patients compared with healthy controls (<0.05). qRT-PCR confirmed miR-7 and miR-21 showes significantly changed levels in plasma samples between IIM patients and healthy controls (<0.05). However, only miR-7 was the sole miRNA lower expression in each IIM patient (<0.05), which is also lower expression in IIM/ILD patients than that without ILD (<0.05). The area under curve (AUC) for distinguishing IIM/ILD patients from IIM without ILD is 0.8978, and the 95% confidence interval is 0.7961 to 0.9995. The receiver operating characteristic (ROC) curve analysis showes miR-7 cutoff value is 0.0063. Further, AUC analysis showes both miR-7 and miR-21 have diagnostic value as biomarkers for IIM patients form health controls; however, miR-7 is more sensitive CONCLUSION: miR-7 is a potential biomarker as a diagnostic indicator for IIM patients and can be used to distinguish IIM/ILD patients from IIM without ILD.
微小RNA（miRNA）已被确定为多种疾病的生物标志物。然而，循环miRNA在特发性炎症性肌病（IIM）诊断中的意义仍不清楚。在本研究中，我们旨在探讨miRNA作为预测IIM和间质性肺病（ILD）患者潜在生物标志物的意义。方法：从43例IIM患者和43例健康人的血浆中分离总RNA。通过miRNA微阵列分析miRNA的表达，并通过qRT-PCR进行验证。结果：微阵列显示，与健康对照相比，IIM患者中发现更多差异表达的循环miRNA（<0.05）。qRT-PCR证实，miR-7和miR-21在IIM患者和健康对照的血浆样本中显示出显著变化的水平（<0.05）。然而，只有miR-7是每个IIM患者中唯一表达较低的miRNA（<0.05），在IIM/ILD患者中的表达也低于无ILD的患者（<0.05）。区分IIM/ILD患者和无ILD的IIM患者的曲线下面积（AUC）为0.8978，95%置信区间为0.7961至0.9995。受试者工作特征（ROC）曲线分析显示，miR-7的截断值为0.0063。此外，AUC分析显示，miR-7和miR-21作为区分IIM患者和健康对照的生物标志物均具有诊断价值；然而，miR-7更敏感。结论：miR-7是IIM患者诊断指标的潜在生物标志物，可用于区分IIM/ILD患者和无ILD的IIM患者。
