通过氟磁共振波谱法在皮肤病局部治疗中对皮质类固醇进行尿液检测。
Urinary detection of corticosteroid in topical treatment of skin disease by F MRS.
作者信息
Sitter Beathe
机构信息
Department of Circulation and Medical Imaging, Faculty of Medicine and Health Sciences, NTNU - Norwegian University of Science and Technology, Trondheim, Norway.
出版信息
MAGMA. 2019 Feb;32(1):157-162. doi: 10.1007/s10334-018-00734-y. Epub 2019 Jan 4.
OBJECTIVE
To investigate if it was feasible to quantify the renal excretion of topically applied corticosteroids by F MRS.
MATERIALS AND METHODS
Five participants, one healthy and four with skin diseases, were treated with ointment containing betamethasone 17-valerate. Urine samples were collected for up to 87 h after the initial application. A sample of ointment mixed with urine served as a study control. Organic fractions were obtained after sample freeze drying, and resolved in deuterated chloroform prior to acquisition of F MR spectra at 470 MHz for typically 8 h.
RESULTS
We detected fluorine signals in 40 of the 62 fractions of organic extracts. The corticosteroid was detected in samples from all patients, ranging from 0.1 to 2.8% of the applied steroid. No fluorine signal was obtained in samples from the healthy volunteer.
DISCUSSION
F MRS can be utilized to detect topically applied corticosteroids in urine. However, more work is required to optimize and control for extraction procedures, complete spectral assignments and reliable quantification.
目的
探讨通过氟磁共振波谱（F MRS）定量局部应用皮质类固醇激素经肾脏排泄的可行性。
材料与方法
5名参与者，1名健康者和4名皮肤病患者，使用含倍他米松17 - 戊酸酯的软膏进行治疗。在首次用药后长达87小时收集尿液样本。一份与尿液混合的软膏样本用作研究对照。样本冷冻干燥后获得有机馏分，并在470 MHz下采集F MR光谱前溶解于氘代氯仿中，通常采集8小时。
结果
在62份有机提取物馏分中的40份检测到氟信号。在所有患者的样本中均检测到皮质类固醇激素，占所用类固醇的0.1%至2.8%。健康志愿者的样本中未获得氟信号。
讨论
F MRS可用于检测尿液中局部应用的皮质类固醇激素。然而，需要更多工作来优化和控制提取程序、完成光谱归属以及进行可靠的定量分析。
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