Adnan Atif, Rakha Allah, Nazir Shahid, Khan Muhammad Farhan, Hadi Sibte, Xuan Jinfeng
Department of Forensic Genetics and Biology, School of Forensic Medicine, China Medical University, Shenyang, 110122, People's Republic of China.
Department of Forensic Sciences, University of Health Sciences Lahore, Lahore, 54000, Pakistan.
Int J Legal Med. 2019 May;133(3):799-802. doi: 10.1007/s00414-018-01997-9. Epub 2019 Jan 4.
Y-chromosomal short tandem repeats (Y-STRs) are commonly used to study population histories, discover ancestral relationships, and identify males for criminal justice purposes. Y-STRs being largely in forensic use have low haplotype diversity in some populations and cannot discriminate between paternal male relatives. Rapidly mutating Y-STRs (RM Y-STRs) were breakthrough and have been paid much attention. A set of 13 rapidly mutating (RM) Y-STRs (DYF387S1, DYF399S1, DYF403S1a/b1/b2, DYF404S1, DYS449, DYS518, DYS526I/II, DYS547, DYS570, DYS576, DYS612, DYS626, and DYS627) typically reveals higher haplotype diversities than the commercially available Y-STR sets and allows differentiating male relatives for which commercial Y-STR sets are usually not informative. Here, we amplified the 13 RM Y-STRs in 168 (37 Sindhi and 131 Punjabi) individuals from Pakistani population, which is characterized by high rates of endogamy. The haplotype diversity and discrimination capacity were 1. Allelic frequencies ranged from 0.0060 to 0.5060, while gene diversity ranged from 0.6759 (DYS526a) to 0.9937 (DYF399S1). A total 319 different alleles were observed. Results of our study showed that RM Y-STRs provided substantially stronger discriminatory power in Pakistani populations.
Y染色体短串联重复序列（Y-STR）常用于研究群体历史、发现祖先关系以及为刑事司法目的识别男性个体。由于Y-STR在法医学中广泛应用，在某些群体中其单倍型多样性较低，无法区分父系男性亲属。快速突变的Y-STR（RM Y-STR）是一项突破，备受关注。一组13个快速突变的（RM）Y-STR（DYF387S1、DYF399S1、DYF403S1a/b1/b2、DYF404S1、DYS449、DYS518、DYS526I/II、DYS547、DYS570、DYS576、DYS612、DYS626和DYS627）通常比市售的Y-STR组显示出更高的单倍型多样性，并且能够区分商业Y-STR组通常无法提供信息的男性亲属。在此，我们在来自巴基斯坦人群的168名个体（37名信德族和131名旁遮普族）中扩增了这13个RM Y-STR，该人群以内婚率高为特征。单倍型多样性和鉴别能力为1。等位基因频率范围为0.0060至0.5060，而基因多样性范围为0.6759（DYS526a）至0.9937（DYF399S1）。共观察到319个不同的等位基因。我们的研究结果表明，RM Y-STR在巴基斯坦人群中提供了更强的鉴别力。
