自噬体的相关光镜与电镜观察
Correlative Light and Electron Microscopy of Autophagosomes.
作者信息
Gudmundsson Sigurdur, Kahlhofer Jenny, Baylac Nastassia, Kallio Katri, Eskelinen Eeva-Liisa
机构信息
Molecular and Integrative Biosciences Research Program, University of Helsinki, Helsinki, Finland.
Division of Cell Biology, Biocenter, Medical University of Innsbruck, Innsbruck, Austria.
出版信息
Methods Mol Biol. 2019;1880:199-209. doi: 10.1007/978-1-4939-8873-0_12.
Live-cell imaging has been widely used to study autophagosome biogenesis and maturation. When combined with correlative electron microscopy, this approach can be extended to reveal ultrastructural details in three dimensions. The resolution of electron microscopy is needed when membrane contact sites and tubular connections between organelles are studied.
活细胞成像已被广泛用于研究自噬体的生物发生和成熟。当与相关电子显微镜相结合时，这种方法可以扩展到揭示三维超微结构细节。在研究细胞器之间的膜接触位点和管状连接时，需要电子显微镜的分辨率。
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