Jung Minkyo, Choi Hyosun, Kim Jaekwang, Mun Ji Young
Neural Circuit Research Group, Korea Brain Research Institute, Daegu 41062, Korea.
BK21 Plus Program, Department of Senior Healthcare, Graduate School, Eulji University, Daejeon 34824, Korea.
Materials (Basel). 2020 Sep 29;13(19):4336. doi: 10.3390/ma13194336.
Propionic acid is a metabolite of the microbiome and can be transported to the brain. Previous data show that propionic acid changes mitochondrial biogenesis in SH-SY5Y cells and induces abnormal autophagy in primary hippocampal neurons. Maintaining mitochondrial function is key to homeostasis in neuronal cells, and mitophagy is the selective autophagy involved in regulating mitochondrial quality. Monitoring mitophagy though light microscopy or conventional transmission electron microscopy separately is insufficient because phases of mitophagy, including autophagosome and autolysosome in nano-resolution, are critical for studies of function. Therefore, we used correlative light and electron microscopy to investigate mitochondrial quality in SH-SY5Y cells after propionic acid treatment to use the advantages of both techniques. We showed, with this approach, that propionic acid induces mitophagy associated with mitochondrial quality.
丙酸是微生物群的一种代谢产物，可被转运至大脑。先前的数据表明，丙酸会改变SH-SY5Y细胞中的线粒体生物合成，并诱导原代海马神经元发生异常自噬。维持线粒体功能是神经元细胞内稳态的关键，而线粒体自噬是参与调节线粒体质量的选择性自噬。单独通过光学显微镜或传统透射电子显微镜监测线粒体自噬是不够的，因为线粒体自噬的各个阶段，包括纳米分辨率下的自噬体和自溶酶体，对于功能研究至关重要。因此，我们使用相关光电子显微镜来研究丙酸处理后的SH-SY5Y细胞中的线粒体质量，以利用这两种技术的优势。通过这种方法，我们发现丙酸会诱导与线粒体质量相关的线粒体自噬。
