Hamm G, Wozniak K D
Klinik und Poliklinik für Hautkrankheiten, Bereiches Medizin, Martin-Luther-Universität Halle-Wittenberg, Halle, Saale.
Arch Dermatol Res. 1988;280(7):416-9. doi: 10.1007/BF00429980.
The amount of bullous pemphigoid antigen (BPA) in normal human skin on different areas of the body and in bodies of different ages was determined by making endpoint titer estimations using the indirect immunofluorescence technique. Two sera with bullous pemphigoid antibodies were tested with 36 specimens of normal human skin from six cadavers. The greatest expression of BPA was found in plantar sites, whereas the lowest endpoint titers were seen in flexural arm biopsy specimens. Age-dependent differences in BPA expression were detected as well. The oldest individuals tested produced the highest endpoint titers in contrast to the lowest endpoint titers in the youngest. This study suggests that the amount of BPA depends not only on the body region where the biopsy specimen is taken but also on the age of the donor. This is important to know in connection with indirect immunofluorescence assays for BP antibodies on human skin.
采用间接免疫荧光技术进行终点滴度测定，以确定正常人体不同部位皮肤及不同年龄段尸体中类天疱疮抗原（BPA）的含量。用两份含类天疱疮抗体的血清检测了来自六具尸体的36份正常人皮肤标本。发现BPA在足底部位表达最高，而在屈侧手臂活检标本中终点滴度最低。还检测到了BPA表达的年龄依赖性差异。与最年轻个体的最低终点滴度相比，检测的最年长者产生了最高的终点滴度。这项研究表明，BPA的含量不仅取决于活检标本采集的身体部位，还取决于供体的年龄。这对于人体皮肤BP抗体的间接免疫荧光检测而言很重要。
