获得性大疱性表皮松解症抗原和大疱性类天疱疮抗原的解剖分布及免疫学特征。

Anatomical distribution and immunological characteristics of epidermolysis bullosa acquisita antigen and bullous pemphigoid antigen.

作者信息

Furue M, Iwata M, Tamaki K, Ishibashi Y

出版信息

Br J Dermatol. 1986 Jun;114(6):651-9. doi: 10.1111/j.1365-2133.1986.tb04872.x.

DOI:10.1111/j.1365-2133.1986.tb04872.x

Abstract

A Japanese patient with epidermolysis bullosa acquisita (EBA) was autopsied, and direct immunofluorescence (DIF) testing was performed. Using this patient's serum (EBA serum) and three bullous pemphigoid (BP) sera, the anatomical distribution and immunological characteristics of EBA antigen and BP antigen were investigated by indirect immunofluorescence (IIF). EBA antigen showed the same anatomical distribution as BP antigen in DIF and IIF studies; both antigens were limited to the skin, tongue, oesophagus, trachea, cornea and bladder. EBA antigen was located on the dermal side of both NaCl and PBS-separated skin, whereas BP antigen was limited to the epidermal side. Ethanol fixation abrogated the antigenic stability of BP antigen, but not that of EBA antigen. No difference was found when acetone or formalin fixation was used. The separation methods and prefixation in ethanol could be useful techniques applicable to the classification of the bullous disorders which manifest circulating anti-BMZ antibodies.

摘要

对一名获得性大疱性表皮松解症（EBA）的日本患者进行了尸检，并进行了直接免疫荧光（DIF）检测。使用该患者的血清（EBA血清）和三种大疱性类天疱疮（BP）血清，通过间接免疫荧光（IIF）研究了EBA抗原和BP抗原的解剖分布及免疫特性。在DIF和IIF研究中，EBA抗原显示出与BP抗原相同的解剖分布；两种抗原均局限于皮肤、舌、食管、气管、角膜和膀胱。EBA抗原位于经氯化钠和磷酸盐缓冲液分离的皮肤的真皮侧，而BP抗原局限于表皮侧。乙醇固定消除了BP抗原的抗原稳定性，但未消除EBA抗原的抗原稳定性。使用丙酮或福尔马林固定时未发现差异。乙醇中的分离方法和预固定可能是适用于对表现出循环抗基底膜带（BMZ）抗体的大疱性疾病进行分类的有用技术。