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Constitutive and inducible expression of cloned human interferon-b1 gene in HeLa cells through an episomal eukaryotic vector.

作者信息

Giannì M, Gallina A, Barbanti-Brodano G, Milanesi G

机构信息

Istituto di Genetica Biochimica ed Evoluzionistica CNR, Pavia, Italy.

出版信息

Biochem Biophys Res Commun. 1988 Dec 30;157(3):1175-81. doi: 10.1016/s0006-291x(88)80998-7.

DOI:10.1016/s0006-291x(88)80998-7
PMID:3061379
Abstract

An EcoRI fragment (1.83 Kb) of human DNA containing B1-interferon coding sequences was cloned in the episomal eukaryotic vector pBK-TK. The recombinant plasmid was transfected into thymidine kinase-deficient HeLa cells. Thymidine kinase-positive transformants were obtained and the cells were found to express B-interferon both constitutively and after induction with Newcastle Disease Virus or poly(rI)-poly(rC). The recombinant plasmid was present in stably transformed HeLa cells in an episomal state in hundreds of copies per cell.

摘要

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