Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610065, Sichuan, People's Republic of China.
Institute of Soil and Groundwater Pollution Control of Sichuan Academy of Environmental Sciences, Chengdu, 610065, Sichuan, People's Republic of China.
Environ Sci Pollut Res Int. 2019 Mar;26(7):6388-6398. doi: 10.1007/s11356-018-4042-3. Epub 2019 Jan 7.
This study investigated the removal and translocation mechanism of cadmium (Cd) by Oudemansiella radicata (O. radicata) in mushroom-soil rhizosphere and the fruiting body of mushroom. For this, the biomass, physiochemical parameters, and Cd distribution of O. radicata were examined in the soil spiked with 0, 10, 20, and 30 mg kg Cd. The soil microecology and the Cd fractionation in the soil rhizosphere were also measured. Results showed that, O. radicata possesses high capability to tolerate Cd, although its surface phenotypic structure was influenced by high concentrations of Cd. The observed concentrations of Cd in O. radicata were in the following order: root (the part of stipe in soil) > pileus > stipe. The presence of Cd led to an increase in the production of antioxidant enzymes and glutathione (GSH). These results suggested that antioxidant enzymes and GSH assisted detoxification and accumulation of Cd within the mushroom. Meanwhile, in the soil rhizosphere, the concentrations of oxalic, citric, and malic acids were enhanced with the treatment of Cd, indicating that the production of these acids was closely related to the presence of Cd in soils. Additionally, the proportion of acid-soluble Cd was increased and the soil microecology (microbial counts, urease, and acid phosphatase activities) also enhanced with the inoculation of O. radicata. Overall, this study demonstrated that O. radicata is a promising candidate for the remediation of Cd-contaminated soil.
本研究调查了草菇（Oudemansiella radicata）在蘑菇土壤根际和子实体中去除和迁移镉（Cd）的机制。为此，在土壤中添加 0、10、20 和 30mgkg Cd 的情况下，研究了 O. radicata 的生物量、理化参数和 Cd 分布。还测量了土壤微生态学和土壤根际中 Cd 的形态。结果表明，尽管 O. radicata 的表面表型结构受到高浓度 Cd 的影响，但它具有高耐 Cd 的能力。在 O. radicata 中观察到的 Cd 浓度顺序为：根（土壤中菌柄的一部分）>菌盖>菌柄。Cd 的存在导致抗氧化酶和谷胱甘肽（GSH）的产生增加。这些结果表明，抗氧化酶和 GSH 有助于蘑菇体内 Cd 的解毒和积累。同时，在土壤根际中，随着 Cd 的处理，草酸、柠檬酸和苹果酸的浓度增加，表明这些酸的产生与土壤中 Cd 的存在密切相关。此外，随着 O. radicata 的接种，酸溶性 Cd 的比例增加，土壤微生物（微生物计数、脲酶和酸性磷酸酶活性）也增强。总的来说，本研究表明，草菇是一种很有前途的修复 Cd 污染土壤的候选物种。
