Key Laboratory for Bio-resources and Eco-environment of Education Ministry, College of Life Science, Sichuan University, Chengdu, 610064, China.
World J Microbiol Biotechnol. 2012 Apr;28(4):1727-37. doi: 10.1007/s11274-011-0983-0. Epub 2011 Dec 24.
In this study, the effects of siderophores produced by six bacteria on mycelium growth, Cd and Pb accumulation, lipid peroxidation, protein content and antioxidant enzyme in Oudemansiella radicata were investigated in Cd and Pb-containing liquid medium. The results showed that inoculation with siderophore-containing filtrates (SCF) partly enhanced the growth of O. radicata after 15 days, with 0.8-32.4% biomass increase for Cd and 0.7-20.8% for Pb compared to control(s), which lacked siderophore. The maximum enhancement for accumulation were found to be confined to Bacillus sp. FFQ2(s) (26.5%) for Cd and Pseudomonas sp. CY63(s) (158.9%) for Pb. A significant decrease in MDA content indicated that lipid peroxidation in O. radicata was alleviated by siderophores. Besides, antioxidant enzyme SOD and POD activities also displayed obviously decrease in SCF-treated mycelium compared to control(s) treatment, while CAT activity did not present significant change. Protein level in O. radicata treated by SCF increased from 0.3 to 138.0% for Cd and from 10.9 to 107.1% for Pb compared to control(s). Therefore, the present work suggests that microbial siderophores can reduce the toxicity of metals to mycelium and then alleviate heavy metals-inducing oxidative stress in O. radicata.
在这项研究中，研究了六种细菌产生的铁载体对含镉和铅的液体培养基中糙皮侧耳菌丝体生长、镉和铅积累、脂质过氧化、蛋白质含量和抗氧化酶的影响。结果表明，与缺乏铁载体的对照相比，接种含铁载体滤液（SCF）后，糙皮侧耳在 15 天后部分促进了生长，镉的生物量增加了 0.8-32.4%，铅的生物量增加了 0.7-20.8%。发现最大的积累增强仅限于芽孢杆菌 FFQ2（26.5%）对 Cd 和假单胞菌 CY63（158.9%）对 Pb。丙二醛含量的显著降低表明铁载体缓解了糙皮侧耳的脂质过氧化。此外，与对照处理相比，SCF 处理的菌丝体中的抗氧化酶 SOD 和 POD 活性也明显降低，而 CAT 活性没有显著变化。与对照相比，SCF 处理的糙皮侧耳中的蛋白质水平从 Cd 的 0.3 增加到 138.0%，从 Pb 的 10.9 增加到 107.1%。因此，本研究表明，微生物铁载体可以降低金属对菌丝体的毒性，从而减轻重金属对糙皮侧耳的诱导氧化应激。
