Hoos P C, Olson G E
Department of Cell Biology, Vanderbilt University, Nashville, Tennessee 37232.
Biol Reprod. 1988 Aug;39(1):131-40. doi: 10.1095/biolreprod39.1.131.
A 23 kDa polypeptide has been identified on the flagellum of sperm obtained from the cauda epididymis of the golden hamster. A monospecific antiserum to the 23 kDa hamster polypeptide was prepared and used to study its distribution on sperm, in the epididymis, and in epididymal fluid. In the cauda, the polypeptide is found on the midpiece and endpiece of the sperm tail, in detergent extracts of sperm, and in epididymal luminal fluid-enriched fractions. It is not present on sperm or in luminal fluid-enriched fractions from the caput epididymis. Immunocytochemical staining of epididymal tissue has demonstrated the 23 kDa polypeptide in the Golgi region of the principal cells of the proximal cauda and on sperm in the tubules of this segment and in tubules distal to it. Antiserum to the 23 kDa golden hamster polypeptide cross-reacts with sperm from rats and Chinese hamsters, but not with sperm from rabbits, cattle, mice, and guinea pigs. The antigen is localized to the tail of sperm obtained from the cauda of the rat and from the distal caput of the Chinese hamster. Immunoblots of detergent extracts of sperm and luminal fluid-enriched fractions from these two species reveal a 26 dKa polypeptide that is immunologically related to the golden hamster polypeptide.
在从金黄仓鼠附睾尾部获取的精子鞭毛上鉴定出一种23 kDa的多肽。制备了针对该23 kDa仓鼠多肽的单特异性抗血清，并用于研究其在精子、附睾以及附睾液中的分布。在附睾尾部，该多肽存在于精子尾部的中段和末段、精子的去污剂提取物以及富含附睾管腔液的组分中。它不存在于附睾头部的精子或富含管腔液的组分中。附睾组织的免疫细胞化学染色显示，在附睾近尾部主细胞的高尔基体区域以及该节段和其远端小管中的精子上存在23 kDa的多肽。针对23 kDa金黄仓鼠多肽的抗血清与大鼠和中国仓鼠的精子发生交叉反应，但与兔、牛、小鼠和豚鼠的精子不发生交叉反应。该抗原定位于从大鼠附睾尾部和中国仓鼠附睾远端头部获取的精子尾部。来自这两个物种的精子去污剂提取物和富含管腔液组分的免疫印迹显示出一种26 kDa的多肽，它与金黄仓鼠多肽具有免疫相关性。
