1Laboratory of Cell Biology, CA Biomedicine of Infectious and Parasitic Diseases, C.I.R. 'Dr. Hideyo Noguchi', University Autonomous of Yucatan, Merida, Mexico.
2Department of Animal Health and Preventive Medicine, Faculty of Veterinary Medicine and Zoothecnics, Campus of Biological and Agropecuary Sciences, University Autonomous of Yucatan, Merida, Mexico.
Vector Borne Zoonotic Dis. 2019 Aug;19(8):619-624. doi: 10.1089/vbz.2018.2385. Epub 2019 Jan 7.
There is little information about in wild felids, even when these species have been associated with cases of toxoplasmosis in humans. In this study, samples of serum and whole blood were collected from 42 felids from 10 different species, in 4 Mexican zoos. Stool samples from 36 animals were also collected, corresponding to 82% of the felids included in the study. Stool samples were used for the search of oocysts by light field microscopy and PCR. Serum samples were analyzed by indirect immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA) and indirect fluorescent antibody test (IFAT). DNA samples were purified from whole blood and stool for the amplification of a fragment of the SAG1 gene of by a nested PCR (nPCR). The seroprevalence of IgG anti--specific antibodies by means of the ELISA was 100% (42/42) and 52.4% (22/42) by IFAT. The titers obtained varied from 1:80 to 1:2560. DNA of was detected in 9.5% (4/42) of the blood samples by using nPCR. No oocysts were observed in the stool samples analyzed by light field microscopy. However, the DNA of the parasite was identified in 14.3% (5/35) of the stool samples evaluated. These results indicate a high prevalence of in the studied populations of wild felids in captivity, with evidence of parasitemia and elimination of few oocysts even in adult hosts.
关于野生猫科动物中的感染情况，信息很少，即使这些物种与人类中的弓形虫病病例有关。在这项研究中，从墨西哥的 4 个动物园的 10 个不同物种的 42 只猫科动物中采集了血清和全血样本。还收集了 36 只动物的粪便样本，占研究中包括的猫科动物的 82%。粪便样本用于通过光场显微镜和 PCR 搜索卵囊。血清样本通过间接免疫球蛋白 G（IgG）酶联免疫吸附试验（ELISA）和间接荧光抗体试验（IFAT）进行分析。从全血和粪便中提取 DNA 样本，通过巢式 PCR（nPCR）扩增 SAG1 基因的片段。通过 ELISA 检测 IgG 抗-特异性抗体的血清阳性率为 100%（42/42），IFAT 为 52.4%（22/42）。获得的效价从 1:80 到 1:2560 不等。使用 nPCR 在 9.5%（4/42）的血液样本中检测到的 DNA。通过光场显微镜分析的粪便样本中未观察到卵囊。然而，在评估的 35 份粪便样本中，有 14.3%（5/35）鉴定出寄生虫的 DNA。这些结果表明，在圈养的野生猫科动物研究人群中，的流行率很高，有寄生虫血症和少数卵囊排出的证据，即使在成年宿主中也是如此。
