Silva J C, Ogassawara S, Adania C H, Ferreira F, Gennari S M, Dubey J P, Ferreira-Neto J S
Departamento de Medicina Veterinária Preventiva e Saúde Animal (VPS), Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP 05508-000, Brazil.
Vet Parasitol. 2001 Dec 13;102(3):217-24. doi: 10.1016/s0304-4017(01)00523-4.
Seroprevalence of Toxoplasma gondii was determined in 865 captive neotropical felids from 20 states from Brazil, sampled from September 1995 to April 1997. Sera were tested by the modified agglutination test (MAT) using formalin-fixed whole tachyzoites and mercaptoethanol. Antibodies (MAT> or =1:20) to T. gondii were found in 472 of 865 (54.6%) cats: in 45 of 99 (45.9%) jaguarundis (Herpailurus yagouaroundi), in 97 of 168 (57.7%) ocelots (Leopardus pardalis), in 68 of 131 (51.9%) oncillas (L. tigrinus), in 35 of 63 (55.5%) margays (L. wiedii), in 1 of 8 (12.5%) Pampas-cat (Oncifelis colocolo), in 9 of 12 (75.0%) Geoffroys-cat (O. geoffroyi), in 134 of 212 (63.2%) jaguars (Panthera onca), and in 83 of 172 (48.2%) pumas (Puma concolor). Antibody titers were: 1:20 in 27 felids, 1:25 in 142 felids, 1:40 in 6 felids, 1:50 in 292 felids, and > or =1:500 in 5 felids. The high seroprevalence of T. gondii antibodies found in the present study suggested a widespread exposure of neotropical cats to T. gondii in zoos in Brazil. The results warrant an investigation on the mode of exposure and oocyst shedding by neotropical cats.
1995年9月至1997年4月期间，对来自巴西20个州的865只圈养新热带区猫科动物进行了弓形虫血清阳性率检测。血清通过改良凝集试验（MAT）检测，使用甲醛固定的全速殖子和巯基乙醇。在865只猫中的472只（54.6%）检测到针对弓形虫的抗体（MAT≥1:20）：99只虎猫（美洲金猫属虎猫）中的45只（45.9%），168只豹猫（豹猫属豹猫）中的97只（57.7%），131只小斑虎猫（虎猫属小斑虎猫）中的68只（51.9%），63只长尾虎猫（长尾虎猫属长尾虎猫）中的35只（55.5%），8只潘帕斯猫（草原猫属草原猫）中的1只（12.5%），12只乔氏猫（乔氏猫属乔氏猫）中的9只（75.0%），212只美洲豹（豹属美洲豹）中的134只（63.2%），以及172只美洲狮（美洲狮属美洲狮）中的83只（48.2%）。抗体滴度分别为：27只猫为1:20，142只猫为1:25，6只猫为1:40，292只猫为1:50，5只猫≥1:500。本研究中发现的高弓形虫抗体血清阳性率表明，巴西动物园中的新热带区猫科动物广泛接触了弓形虫。这些结果值得对新热带区猫科动物的接触方式和卵囊排出情况进行调查。
