Department of Experimental Immunology, Medical University of Lodz, Pomorska 251, 92-213, Lodz, Poland.
Department of Old Age Psychiatry and Psychotic Disorders, Medical University of Lodz, Lodz, Poland.
Psychiatry Res. 2019 Feb;272:540-550. doi: 10.1016/j.psychres.2018.12.138. Epub 2018 Dec 28.
Increasing evidence suggests that in addition to neurochemical abnormalities, various immunological alterations are related to the pathogenesis of schizophrenia. Toll-like receptors (TLRs) actively mediate immune/inflammatory processes and play a pivotal role in damage/danger recognizing. Therefore, the aim of this study was to compare the expression of TLRs in peripheral blood mononuclear cells (PBMCs) in schizophrenic patients with those of healthy subjects. It also measures the metabolic status of the study subjects. Twenty-seven adult European Caucasian patients with paranoid schizophrenia and twenty-nine healthy volunteers were included in this prospective study. qRT-PCR assessed TLR mRNA expression levels. Body composition was measured using two methods: bioimpedance analysis (BIA) and dual-energy X-ray absorptiometry (DXA). The TLR1, TLR2, TLR4, TLR6, and TLR9 expression were down-regulated, in opposite to TLR3 and TLR7 which manifested higher expression in patients with schizophrenia. TLR5 and TLR8 mRNAs did not differ between groups. TLR mRNA expression was highly correlated. Decreased TLR expression may protect against excessive cell stimulation via exogenous and/or endogenous ligands, and may be recognized as a counterbalancing mechanism limiting the excessive development of inflammation.
越来越多的证据表明，除了神经化学异常外，各种免疫改变与精神分裂症的发病机制有关。 Toll 样受体 (TLRs) 积极介导免疫/炎症过程，并在损伤/危险识别中发挥关键作用。因此，本研究的目的是比较精神分裂症患者与健康受试者外周血单个核细胞 (PBMC) 中 TLRs 的表达。它还测量了研究对象的代谢状况。本前瞻性研究纳入了 27 名成年欧洲白种人偏执型精神分裂症患者和 29 名健康志愿者。qRT-PCR 评估 TLR mRNA 表达水平。使用两种方法测量身体成分：生物阻抗分析 (BIA) 和双能 X 射线吸收法 (DXA)。TLR1、TLR2、TLR4、TLR6 和 TLR9 的表达下调，而 TLR3 和 TLR7 的表达则升高。TLR5 和 TLR8 的 mRNA 在两组之间没有差异。TLR mRNA 表达高度相关。降低的 TLR 表达可能通过外源性和/或内源性配体来防止过度的细胞刺激，并且可以被认为是一种平衡机制，限制炎症的过度发展。
