ACP 与 MDP 的相互作用及其对牙本质粘结性能的影响。
Interaction of ACP and MDP and its effect on dentin bonding performance.
机构信息
Department of Prosthodontics, Hospital of Stomatology Affiliated to Zhejiang University School of Medicine, Yan'an Road 395,Hangzhou 310006, Zhejiang, China; Key Laboratory for Oral Biomedical Research of Zhejiang Province, Hangzhou 310006, Zhejiang, China.
Department of Prosthodontics, Hospital of Stomatology Affiliated to Zhejiang University School of Medicine, Yan'an Road 395,Hangzhou 310006, Zhejiang, China; Key Laboratory for Oral Biomedical Research of Zhejiang Province, Hangzhou 310006, Zhejiang, China.
出版信息
J Mech Behav Biomed Mater. 2019 Mar;91:301-308. doi: 10.1016/j.jmbbm.2018.12.017. Epub 2018 Dec 18.
OBJECTIVES
The study investigated the solid reactants of amorphous calcium phosphate (ACP) nanoparticles with 10-methacryloyloxydecyl dihydrogen phosphate (MDP) in ethanol-aqueous solution, and the effect of ACP-MDP suspension as a novel primer on dentin micro-tesnisl bond strengths (MTBS) prior to application of self-etch adhesives.
MATERIALS AND METHODS
The ACP nanoparticles were synthesized. The solid reactants of ACP nanoparticles with MDP ethanol-aqueous solution were analyzed by TEM, SEM, XRD and AFM. After the ACP-MDP complexes were attacked by alkaline, acidic and self-etch adhesive challenges, they were analyzed with TEM. The polished mid-coronal dentin surfaces of six third molars were treated with ACP-MDP suspension, 5% MDP solution or not (served as control), and thoroughly water-sprayed. The pretreated dentin surfaces were applied with a self-etch adhesive (Clearfil S3 Bond) and placed with a composite resin (Clearfil Majesty). After 24 h, the resin-bonded samples were cut into multiple beams before the MTBS tests were performed. The failure modes were determined by a stereo-microscopy. The pretreated dentin surfaces were analyzed by SEM. The resin-dentin interfaces were analyzed with TEM.
RESULTS
The ACP nanoparticles in the MDP ethanol-aqueous solution at certain molar ratio of Ca:MDP (0.12:1) could self-assemble into ACP-MDP complexes consisting of nanolayering structures. The ACP-MDP self-assembly went from ACP nanoparticles, beaded structures, and twig-like structures to networks. The ACP-MDP complexes can be resistant to adhesive challenge, but not to alkaline and acidic challenges. ACP-MDP complexes possessed a similar modulus of elasticity to dentin, and were stable at least for 3 months. Furthermore, the dentin surfaces treated with ACP-MDP suspension could significantly increase the dentin MTBS when compared with control and those treated with 5% MDP solution (P < 0.05).
CONCLUSION
The ACP-MDP suspension at certain molar ratio of Ca:MDP (0.12:1) could be self-assembled into ACP-MDP complexes consisting of nanolayering structures, and could be used as a novel primer to greatly improve the dentin bond strengths.
目的
本研究探讨了在乙醇-水溶液中无定形磷酸钙（ACP）纳米颗粒与 10-甲基丙烯酰氧基癸基二氢磷酸酯（MDP）的固相反应物，以及在应用自酸蚀粘结剂之前，将 ACP-MDP 悬浮液作为新型底漆应用于牙本质微拉伸粘结强度（MTBS）的效果。
材料与方法
合成 ACP 纳米颗粒。通过 TEM、SEM、XRD 和 AFM 分析 ACP 纳米颗粒与 MDP 乙醇-水溶液的固相反应物。在碱性、酸性和自酸蚀粘结剂的挑战下对 ACP-MDP 复合物进行攻击后，用 TEM 进行分析。用 ACP-MDP 悬浮液、5% MDP 溶液或不处理（作为对照）处理 6 颗第三磨牙的中冠状牙本质表面，并用去离子水彻底喷雾。用自酸蚀粘结剂（Clearfil S3 Bond）预处理牙本质表面，并用复合树脂（Clearfil Majesty）填充。24 小时后，将树脂粘结样本切割成多个横梁，然后进行 MTBS 测试。用立体显微镜确定失效模式。用 SEM 分析预处理牙本质表面。用 TEM 分析树脂-牙本质界面。
结果
在一定 Ca：MDP 摩尔比（0.12：1）的 MDP 乙醇-水溶液中，ACP 纳米颗粒可以自组装成由纳米层状结构组成的 ACP-MDP 复合物。ACP-MDP 自组装从 ACP 纳米颗粒、珠状结构和树枝状结构到网络结构。ACP-MDP 复合物可以抵抗粘结剂的挑战，但不能抵抗碱性和酸性的挑战。ACP-MDP 复合物的弹性模量与牙本质相似，至少在 3 个月内是稳定的。此外，与对照组和用 5% MDP 溶液处理的牙本质表面相比，用 ACP-MDP 悬浮液处理的牙本质表面可以显著提高牙本质的 MTBS（P<0.05）。
结论
在一定 Ca：MDP 摩尔比（0.12：1）的 MDP 乙醇-水溶液中，ACP-MDP 可以自组装成由纳米层状结构组成的 ACP-MDP 复合物，并可作为新型底漆，显著提高牙本质粘结强度。
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