Departments of Medicine, University of California, Los Angeles, Los Angeles, CA 90095.
Departments of Medicine, University of California, Los Angeles, Los Angeles, CA 90095
J Lipid Res. 2019 Mar;60(3):450-455. doi: 10.1194/jlr.S090316. Epub 2019 Jan 7.
Secreted proteins serve as crucial mediators of many physiology processes, and beginning with the discovery of insulin, studies have revealed numerous context-specific regulatory networks across various cell types. Here, we review "omics" approaches to deconvolute the complex milieu of proteins that are released from the cell. We emphasize a novel "systems genetics" approach our laboratory has developed to investigate mechanisms of tissue-tissue communication using population-based datasets. Finally, we highlight potential future directions for these studies, discuss several caveats, and propose new ways to investigate modes of endocrine communication.
分泌蛋白作为许多生理过程的关键介质，从胰岛素的发现开始，研究已经揭示了各种细胞类型中许多特定于上下文的调节网络。在这里，我们回顾了“组学”方法，以剖析从细胞中释放的复杂的蛋白质环境。我们强调了我们实验室开发的一种新的“系统遗传学”方法，用于使用基于人群的数据集研究组织间通讯的机制。最后，我们强调了这些研究的潜在未来方向，讨论了一些注意事项，并提出了新的方法来研究内分泌通讯的模式。
