Biochemistry Department, University of Wisconsin-Madison, Madison, WI, USA.
Adv Exp Med Biol. 2018;1105:43-58. doi: 10.1007/978-981-13-2200-6_5.
We briefly review current technology for structure-function investigations of biological macromolecules in solution by nuclear magnetic resonance spectroscopy, which enable hybrid methods. An advantage of NMR is that biomolecules can be studied at atomic resolution under near physiological conditions where they are dynamically active. We outline stable isotope labeling strategies, NMR data collection methodology, and procedures for data analysis leading to structure-function information. We discuss issues related to NMR software and data deposition.
我们简要回顾了当前通过核磁共振波谱技术研究溶液中生物大分子的结构-功能的技术，这些技术使混合方法成为可能。核磁共振的一个优势是，生物分子可以在接近生理条件下动态活跃的情况下，以原子分辨率进行研究。我们概述了稳定同位素标记策略、NMR 数据采集方法以及导致结构-功能信息的数据分析程序。我们讨论了与 NMR 软件和数据存储相关的问题。
