ACS Appl Mater Interfaces. 2019 Jan 30;11(4):3690-3703. doi: 10.1021/acsami.8b17533. Epub 2019 Jan 18.
Gold nanorods (GNRs) and doxorubicin (DOX) were loaded into the lumen of halloysite nanotubes (HNTs) via a rapid synthesis process (2 min) and physical adsorption. The targeting molecules of folic acid (FA) are then conjugated to HNTs via reactions with bovine serum albumin (BSA). The formation of GNRs in HNTs was verified by different techniques. Au-HNT-DOX@BSA-FA shows a maximum temperature of 26.8 °C rising after 8 min of 808 nm laser irradiation under 0.8 W cm. The functionalized HNTs exhibited stronger chemotherapeutic effect under laser irradiation as the laser could promote the release of DOX and temperature rising. Au-HNT-DOX@BSA-FA-treated MCF-7 cells exhibited a survival rate of 7.4% after laser irradiation. Au-HNT-DOX@BSA-FA treatment does not induce obvious toxicity in blood biochemistry, liver, and kidney function in normal mice. In vivo chemo-photothermal treatment toward 4T1-bearing mice suggested that Au-HNT-DOX@BSA-FA exhibited remarkable tumor-targeted efficiency and good controlled release effect for DOX. Also, the nanoparticles exhibited a rapid photothermal performance and an ability to inhibit the growth of tumors. Because of the synergistic effect of chemical-photothermal therapy, the toxicity of DOX to normal tissues was reduced on the premise of ensuring the same curative effect with a low dosage of 0.32 mg kg. This novel chemo-photothermal therapy nanoplatform provided a safe, rapid, effective, and cheap choice for the treatment of breast tumors both in vitro and in vivo.
金纳米棒（GNRs）和阿霉素（DOX）通过快速合成过程（2 分钟）和物理吸附装载到多孔纳米管（HNTs）的内腔中。然后，通过与牛血清白蛋白（BSA）的反应将叶酸（FA）的靶向分子接枝到 HNTs 上。通过不同的技术验证了 GNRs 在 HNTs 中的形成。Au-HNT-DOX@BSA-FA 在 808nm 激光照射 8 分钟后，在 0.8Wcm 下显示出 26.8°C 的最大温度升高。功能化的 HNTs 在激光照射下表现出更强的化疗效果，因为激光可以促进 DOX 的释放和温度升高。Au-HNT-DOX@BSA-FA 处理的 MCF-7 细胞在激光照射后存活率为 7.4%。Au-HNT-DOX@BSA-FA 处理在正常小鼠的血液生化、肝和肾功能中没有引起明显的毒性。在 4T1 荷瘤小鼠体内的化学-光热治疗表明，Au-HNT-DOX@BSA-FA 表现出显著的肿瘤靶向效率和对 DOX 的良好控制释放效果。此外，该纳米粒子表现出快速的光热性能和抑制肿瘤生长的能力。由于化学-光热治疗的协同作用，在保证相同疗效的前提下，降低了 DOX 对正常组织的毒性，用药剂量低至 0.32mgkg。这种新型的化学-光热治疗纳米平台为体外和体内治疗乳腺癌提供了一种安全、快速、有效且廉价的选择。
