Ackrill A M, Blair G E
Department of Biochemistry, University of Leeds, U.K.
Eur J Cancer Clin Oncol. 1988 Nov;24(11):1745-50. doi: 10.1016/0277-5379(88)90076-4.
The steady-state levels of hamster MHC class I mRNA and cell surface protein were analysed in cells transformed by either adenovirus type 12 (Ad12) or types 2 or 5 (Ad2 or Ad5). All cell lines were oncogenic in new born and adult hamsters. A great reduction in both class I mRNA and protein was observed in Ad12 transformed cells compared to cells transformed by Ad2 or Ad5. Analysis of class I mRNA in solid tumours induced in hamsters by Ad transformed cell lines also showed greatly reduced mRNA levels in tumours induced by Ad12 compared to those induced by Ad2 or Ad5. This suggests that, in the Ad transformed hamster cell system, reduction in the level of MHC class I gene expression is not necessarily associated with tumour formation.
分析了感染12型腺病毒（Ad12）或2型或5型腺病毒（Ad2或Ad5）后转化的细胞中仓鼠MHC I类mRNA和细胞表面蛋白的稳态水平。所有细胞系在新生和成年仓鼠中均具有致癌性。与Ad2或Ad5转化的细胞相比，在Ad12转化的细胞中观察到I类mRNA和蛋白均大幅减少。对Ad转化细胞系诱导的仓鼠实体瘤中I类mRNA的分析也表明，与Ad2或Ad5诱导的肿瘤相比，Ad12诱导的肿瘤中mRNA水平大幅降低。这表明，在Ad转化的仓鼠细胞系统中，MHC I类基因表达水平的降低不一定与肿瘤形成相关。
