• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

脂多糖通过β-连环蛋白与环氧化酶-2之间的正反馈刺激NIH3T3细胞迁移。

Lipopolysaccharide Stimulated the Migration of NIH3T3 Cells Through a Positive Feedback Between β-Catenin and COX-2.

作者信息

Li Xiao-Jun, Huang Feng-Zhen, Wan Yan, Li Yu-Sang, Zhang Wei Kevin, Xi Yang, Tian Gui-Hua, Tang He-Bin

机构信息

Department of Pharmacology, School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan, China.

School of Medicine, Institute of Biochemistry and Molecular Biology, Ningbo University, Ningbo, China.

出版信息

Front Pharmacol. 2018 Dec 19;9:1487. doi: 10.3389/fphar.2018.01487. eCollection 2018.

DOI:10.3389/fphar.2018.01487
PMID:30618773
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6305731/
Abstract

How β-catenin/COX-2 contribute to inflammation-induced fibroblasts migration remains poorly understood. Therefore, in this study, lipopolysaccharide (LPS) was used as a stimulus to accelerate the migration of NIH3T3 cells, which mimicked the tissue repair process. LPS treatment increased the cell migration in concentration-and time-dependent manner. And NS398, a COX-2 inhibitor, inhibited LPS-induced NIH3T3 cells migration. DKK-1, an antagonist of the Wnt/β-catenin signaling, also inhibited that migration. However, TWS119, an inducer of β-catenin via GSK-3β, increased the cell migration. LPS or TWS119 treatment increased COX-2, β-catenin, , and expressions, and that could be attenuated by NS398 or DKK-1 addition. LPS induced the PGE production, and PGE increased the expression and nuclear translocation of β-catenin, while EP2 blocker, AH6809, alleviated those effects. TWS119 increased the luciferase activity in the COX-2 promoter. In conclusion, LPS stimulated the NIH3T3 fibroblasts migration through a positive feedback between β-catenin and COX-2, in which PGE, EP2, , and played as signal molecules.

摘要

β-连环蛋白/COX-2如何促进炎症诱导的成纤维细胞迁移仍不清楚。因此，在本研究中，使用脂多糖（LPS）作为刺激物来加速NIH3T3细胞的迁移，这模拟了组织修复过程。LPS处理以浓度和时间依赖性方式增加细胞迁移。并且，COX-2抑制剂NS398抑制LPS诱导的NIH3T3细胞迁移。Wnt/β-连环蛋白信号拮抗剂DKK-1也抑制该迁移。然而，通过GSK-3β诱导β-连环蛋白的TWS119增加了细胞迁移。LPS或TWS119处理增加了COX-2、β-连环蛋白等的表达，而添加NS398或DKK-1可减弱这种表达。LPS诱导PGE产生，PGE增加β-连环蛋白的表达和核转位，而EP2阻滞剂AH6809减轻了这些作用。TWS119增加了COX-2启动子中的荧光素酶活性。总之，LPS通过β-连环蛋白和COX-2之间的正反馈刺激NIH3T3成纤维细胞迁移，其中PGE、EP2等作为信号分子发挥作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c096/6305731/14b8841e1fb6/fphar-09-01487-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c096/6305731/37cc65bd349b/fphar-09-01487-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c096/6305731/c4f32328b139/fphar-09-01487-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c096/6305731/289b223be2c7/fphar-09-01487-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c096/6305731/8f3cb1e14732/fphar-09-01487-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c096/6305731/ecabee0ea90e/fphar-09-01487-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c096/6305731/5bf2081f4ebf/fphar-09-01487-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c096/6305731/3f4ee3c98a4c/fphar-09-01487-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c096/6305731/2755cd5d4481/fphar-09-01487-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c096/6305731/14b8841e1fb6/fphar-09-01487-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c096/6305731/37cc65bd349b/fphar-09-01487-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c096/6305731/c4f32328b139/fphar-09-01487-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c096/6305731/289b223be2c7/fphar-09-01487-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c096/6305731/8f3cb1e14732/fphar-09-01487-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c096/6305731/ecabee0ea90e/fphar-09-01487-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c096/6305731/5bf2081f4ebf/fphar-09-01487-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c096/6305731/3f4ee3c98a4c/fphar-09-01487-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c096/6305731/2755cd5d4481/fphar-09-01487-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c096/6305731/14b8841e1fb6/fphar-09-01487-g010.jpg

相似文献

1
Lipopolysaccharide Stimulated the Migration of NIH3T3 Cells Through a Positive Feedback Between β-Catenin and COX-2.脂多糖通过β-连环蛋白与环氧化酶-2之间的正反馈刺激NIH3T3细胞迁移。
Front Pharmacol. 2018 Dec 19;9:1487. doi: 10.3389/fphar.2018.01487. eCollection 2018.
2
TGF-beta1 targets the GSK-3beta/beta-catenin pathway via ERK activation in the transition of human lung fibroblasts into myofibroblasts.在人肺成纤维细胞向肌成纤维细胞转变过程中，转化生长因子-β1通过激活细胞外信号调节激酶（ERK）靶向糖原合成酶激酶-3β/β-连环蛋白信号通路。
Pharmacol Res. 2008 Apr;57(4):274-82. doi: 10.1016/j.phrs.2008.02.001. Epub 2008 Feb 9.
3
Synergistic effects of CD44 and TGF-β1 through AKT/GSK-3β/β-catenin signaling during epithelial-mesenchymal transition in liver cancer cells.CD44与转化生长因子-β1在肝癌细胞上皮-间质转化过程中通过AKT/糖原合成酶激酶-3β/β-连环蛋白信号通路产生的协同作用。
Biochem Biophys Res Commun. 2016 Sep 2;477(4):568-574. doi: 10.1016/j.bbrc.2016.06.077. Epub 2016 Jun 16.
4
Interaction between the Wnt/β-catenin signaling pathway and the EMMPRIN/MMP-2, 9 route in periodontitis.牙周炎中 Wnt/β-连环蛋白信号通路与 EMMPRIN/MMP-2、9 途径的相互作用。
J Periodontal Res. 2018 Oct;53(5):842-852. doi: 10.1111/jre.12574. Epub 2018 Jun 14.
5
Sustained Wnt/β-catenin signaling rescues high glucose induction of transforming growth factor-β1-mediated renal fibrosis.持续的 Wnt/β-连环蛋白信号转导挽救高糖诱导的转化生长因子-β1 介导的肾脏纤维化。
Am J Med Sci. 2012 Nov;344(5):374-82. doi: 10.1097/MAJ.0b013e31824369c5.
6
Activation of dickkopf-1 and focal adhesion kinase pathway by tumour necrosis factor α induces enhanced migration of fibroblast-like synoviocytes in rheumatoid arthritis.肿瘤坏死因子α激活Dickkopf-1和粘着斑激酶通路可诱导类风湿关节炎中滑膜成纤维样细胞迁移增强。
Rheumatology (Oxford). 2016 May;55(5):928-38. doi: 10.1093/rheumatology/kev422. Epub 2015 Dec 29.
7
Glycogen synthase kinase-3 beta inhibitor suppresses Porphyromonas gingivalis lipopolysaccharide-induced CD40 expression by inhibiting nuclear factor-kappa B activation in mouse osteoblasts.糖原合酶激酶-3β抑制剂通过抑制核因子-κB 活化抑制牙龈卟啉单胞菌脂多糖诱导的小鼠成骨细胞 CD40 表达。
Mol Immunol. 2012 Aug;52(1):38-49. doi: 10.1016/j.molimm.2012.04.005. Epub 2012 May 14.
8
Therapeutic intervention of proanthocyanidins on the migration capacity of melanoma cells is mediated through PGE2 receptors and β-catenin signaling molecules.原花青素对黑色素瘤细胞迁移能力的治疗干预是通过前列腺素E2受体和β-连环蛋白信号分子介导的。
Am J Cancer Res. 2015 Oct 15;5(11):3325-38. eCollection 2015.
9
Wnt/β-catenin signaling pathway contributes to isoflurane postconditioning against cerebral ischemia-reperfusion injury and is possibly related to the transforming growth factorβ1/Smad3 signaling pathway.Wnt/β-catenin 信号通路有助于异氟醚后处理对脑缺血再灌注损伤的保护作用，其可能与转化生长因子β1/Smad3 信号通路有关。
Biomed Pharmacother. 2019 Feb;110:420-430. doi: 10.1016/j.biopha.2018.11.143. Epub 2018 Dec 5.
10
GSK-3β inhibitor TWS119 attenuates rtPA-induced hemorrhagic transformation and activates the Wnt/β-catenin signaling pathway after acute ischemic stroke in rats.GSK-3β抑制剂TWS119可减轻大鼠急性缺血性卒中后rtPA诱导的出血性转化并激活Wnt/β-连环蛋白信号通路。
Mol Neurobiol. 2016 Dec;53(10):7028-7036. doi: 10.1007/s12035-015-9607-2. Epub 2015 Dec 15.

引用本文的文献

1
Synthesis of a -GFOGER Adamantane-Based Collagen Mimetic Peptide Imbibed in a Hyaluronic Acid Hydrogel for Enhanced Wound Healing.负载于透明质酸水凝胶中的基于α-GFOGER金刚烷的胶原蛋白模拟肽的合成，用于增强伤口愈合
ACS Appl Bio Mater. 2025 Jun 16;8(6):4657-4672. doi: 10.1021/acsabm.4c01895. Epub 2025 Feb 19.
2
Synthesis and characterization of new hexahydroquinoline derivatives and evaluation of their cytotoxicity, intracellular ROS production, and inhibitory effects on inflammatory mediators.新型六氢喹啉衍生物的合成、表征及其细胞毒性、细胞内活性氧生成以及对炎症介质抑制作用的评估
Turk J Chem. 2024 Jul 23;48(4):659-675. doi: 10.55730/1300-0527.3686. eCollection 2024.
3

本文引用的文献

1
High-mobility group box 1 protein orchestrates responses to tissue damage via inflammation, innate and adaptive immunity, and tissue repair.高迁移率族蛋白 B1 通过炎症、先天和适应性免疫以及组织修复来协调对组织损伤的反应。
Immunol Rev. 2017 Nov;280(1):74-82. doi: 10.1111/imr.12601.
2
Inflammation and metabolism in tissue repair and regeneration.组织修复和再生中的炎症和代谢。
Science. 2017 Jun 9;356(6342):1026-1030. doi: 10.1126/science.aam7928. Epub 2017 Jun 8.
3
The dark side of "the force" - lipid nanoparticles enhance the oncogenesis of diethylnitrosamine and result in liver cancer in mice.
Dental Pulp Stem Cells Modulate Inflammasome Pathway and Collagen Deposition of Dermal Fibroblasts.
牙髓干细胞调节皮肤成纤维细胞的炎症小体途径和胶原蛋白沉积。
Cells. 2024 May 14;13(10):836. doi: 10.3390/cells13100836.
4
Network proteomic analysis identifies inter-alpha-trypsin inhibitor heavy chain 4 during early human Achilles tendon healing as a prognostic biomarker of good long-term outcomes.网络蛋白质组学分析鉴定出在人类跟腱早期愈合过程中，α-胰蛋白酶抑制剂重链 4 是一个预后良好的生物标志物。
Front Immunol. 2023 Jul 6;14:1191536. doi: 10.3389/fimmu.2023.1191536. eCollection 2023.
5
Gradual deterioration of fatty liver disease to liver cancer inhibition of AMPK signaling pathways involved in energy-dependent disorders, cellular aging, and chronic inflammation.脂肪肝疾病逐渐恶化为肝癌，抑制参与能量依赖性紊乱、细胞衰老和慢性炎症的AMPK信号通路。
Front Oncol. 2023 Mar 2;13:1099624. doi: 10.3389/fonc.2023.1099624. eCollection 2023.
6
A refractory liver metastatic solid pseudopapillary neoplasm pancreas harbored mutation showed good response to celecoxib: A case report.一例对塞来昔布有良好反应的难治性肝转移实性假乳头状胰腺肿瘤病例报告：携带突变
Front Oncol. 2022 Oct 28;12:1022290. doi: 10.3389/fonc.2022.1022290. eCollection 2022.
7
Enhanced healing of oral chemical burn by inhibiting inflammatory factors with an oral administration of shengFu oil.口服生肤油通过抑制炎症因子促进口腔化学烧伤的愈合。
Front Pharmacol. 2022 Aug 11;13:913098. doi: 10.3389/fphar.2022.913098. eCollection 2022.
8
Investigation of the 3D Printability of Covalently Cross-Linked Polypeptide-Based Hydrogels.共价交联多肽基水凝胶的3D打印适性研究。
ACS Omega. 2022 Feb 28;7(9):7556-7571. doi: 10.1021/acsomega.1c05873. eCollection 2022 Mar 8.
9
Therapeutic candidates for keloid scars identified by qualitative review of scratch assay research for wound healing.通过定性评价划痕试验研究鉴定瘢痕疙瘩的治疗候选物，以促进伤口愈合。
PLoS One. 2021 Jun 18;16(6):e0253669. doi: 10.1371/journal.pone.0253669. eCollection 2021.
10
Nanoparticle-Encapsulated Liushenwan Could Treat Nanodiethylnitrosamine-Induced Liver Cancer in Mice by Interfering With Multiple Critical Factors for the Tumor Microenvironment.纳米颗粒包裹的六神丸可通过干扰肿瘤微环境的多个关键因素治疗小鼠二乙基亚硝胺诱导的肝癌。
Front Pharmacol. 2020 Jul 10;11:1052. doi: 10.3389/fphar.2020.01052. eCollection 2020.
“原力”的阴暗面——脂质纳米颗粒增强二乙基亚硝胺的致癌作用并导致小鼠患肝癌。
Nanomedicine. 2017 Feb;13(2):701-711. doi: 10.1016/j.nano.2016.09.017. Epub 2016 Oct 8.
4
NTS/NTR1 co-expression enhances epithelial-to-mesenchymal transition and promotes tumor metastasis by activating the Wnt/β-catenin signaling pathway in hepatocellular carcinoma.NTS/NTR1共表达通过激活肝细胞癌中的Wnt/β-连环蛋白信号通路增强上皮-间质转化并促进肿瘤转移。
Oncotarget. 2016 Oct 25;7(43):70303-70322. doi: 10.18632/oncotarget.11854.
5
Inhibition of Wnt/β-catenin signaling suppresses bleomycin-induced pulmonary fibrosis by attenuating the expression of TGF-β1 and FGF-2.抑制Wnt/β-连环蛋白信号通路可通过减弱转化生长因子-β1（TGF-β1）和碱性成纤维细胞生长因子-2（FGF-2）的表达来抑制博来霉素诱导的肺纤维化。
Exp Mol Pathol. 2016 Aug;101(1):22-30. doi: 10.1016/j.yexmp.2016.04.003. Epub 2016 Apr 23.
6
Key Role of DAMP in Inflammation, Cancer, and Tissue Repair.损伤相关分子模式在炎症、癌症和组织修复中的关键作用。
Clin Ther. 2016 May;38(5):1017-28. doi: 10.1016/j.clinthera.2016.02.028. Epub 2016 Mar 25.
7
Up-Regulation of the Biosynthesis and Release of Substance P through Wnt/β-Catenin Signaling Pathway in Rat Dorsal Root Ganglion Cells.通过Wnt/β-连环蛋白信号通路上调大鼠背根神经节细胞中P物质的生物合成与释放
PLoS One. 2015 Jun 8;10(6):e0129701. doi: 10.1371/journal.pone.0129701. eCollection 2015.
8
Effect of different chitosan derivatives on in vitro scratch wound assay: a comparative study.不同壳聚糖衍生物对体外划痕实验的影响：比较研究。
Int J Biol Macromol. 2015 May;76:236-41. doi: 10.1016/j.ijbiomac.2015.02.041. Epub 2015 Mar 4.
9
Honokiol inhibits non-small cell lung cancer cell migration by targeting PGE₂-mediated activation of β-catenin signaling.和厚朴酚通过靶向 PGE₂ 介导的β-连环蛋白信号激活抑制非小细胞肺癌细胞迁移。
PLoS One. 2013 Apr 8;8(4):e60749. doi: 10.1371/journal.pone.0060749. Print 2013.
10
High mobility group A1 protein acts as a new target of Notch1 signaling and regulates cell proliferation in T leukemia cells.高迁移率族蛋白 A1 作为 Notch1 信号的新靶点，调节 T 白血病细胞的增殖。
Mol Cell Biochem. 2013 Feb;374(1-2):173-80. doi: 10.1007/s11010-012-1517-2. Epub 2012 Nov 16.