Deng Shuang, Zhou Jian-Lin, Fang Hong-Song, Nie Zhi-Gang, Chen Sen, Peng Hao
Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, China.
Front Physiol. 2018 Dec 11;9:1787. doi: 10.3389/fphys.2018.01787. eCollection 2018.
Glucocorticoids intake has become the most common pathogenic factor for osteonecrosis of the femoral head (ONFH). Annually, tens of millions of patients suffer from pain related to ONFH. Researchers have proposed several underlying mechanisms of ONFH, including osteocyte apoptosis, cell differentiation disorder, and angiogenesis hindrance. Sesamin, isolated from seeds, was reported could affect osteocyte inflammation and differentiation in osteoarthritis and osteoporosis. We investigated the underlying influence of sesamin on ONFH rat model. Fifteen male Sprague-Dawley rats were randomly divided into three groups. The ONFH model group only received the methylprednisolone (MPS) and lipopolysaccharide (LPS) injection to promote the development of ONFH. The sesamin treatment group was injected with sesamin, MPS, and LPS. The control group was untreated. Rats from above groups were sacrificed 4 weeks later. The effect of sesamin on ONFH rats was validated by H&E staining. TUNEL staining showed that femoral head necrosis was attenuated by sesamin. Furthermore, the phosphorylation of Akt was increased and the downstream cellular apoptosis signal pathway was inhibited. Intracellular ROS level was decreased after sesamin treatment. In conclusion, our findings suggest that sesamin protects the femoral head from osteonecrosis by inhibiting ROS-induced osteoblast apoptosis.
糖皮质激素的摄入已成为股骨头坏死（ONFH）最常见的致病因素。每年，数以千万计的患者遭受与ONFH相关的疼痛。研究人员提出了几种ONFH的潜在机制，包括骨细胞凋亡、细胞分化紊乱和血管生成障碍。从种子中分离出的芝麻素据报道可影响骨关节炎和骨质疏松症中的骨细胞炎症和分化。我们研究了芝麻素对ONFH大鼠模型的潜在影响。15只雄性Sprague-Dawley大鼠被随机分为三组。ONFH模型组仅接受甲基泼尼松龙（MPS）和脂多糖（LPS）注射以促进ONFH的发展。芝麻素治疗组注射芝麻素、MPS和LPS。对照组未接受治疗。4周后处死上述各组大鼠。通过苏木精-伊红（H&E）染色验证芝麻素对ONFH大鼠的作用。TUNEL染色显示芝麻素减轻了股骨头坏死。此外，Akt的磷酸化增加，下游细胞凋亡信号通路受到抑制。芝麻素处理后细胞内活性氧（ROS）水平降低。总之，我们的研究结果表明，芝麻素通过抑制ROS诱导的成骨细胞凋亡来保护股骨头免受坏死。
