Kim Young-Saeng, Kim Jin-Ju, Park Seong-Im, Diamond Spencer, Boyd Joseph S, Taton Arnaud, Kim Il-Sup, Golden James W, Yoon Ho-Sung
Research Institute of Ulleung-do and Dok-do, Kyungpook National University, Daegu, South Korea.
Department of Biology, College of Natural Sciences, Kyungpook National University, Daegu, South Korea.
Front Plant Sci. 2018 Dec 11;9:1848. doi: 10.3389/fpls.2018.01848. eCollection 2018.
Cyanobacterial 2-Cys peroxiredoxin (thioredoxin peroxidase, ) comprises a family of thiol antioxidant enzymes critically involved in cell survival under oxidative stress. In our previous study, a putative was identified using a proteomics analysis of rice ( L. , ) seedlings exposed to oxidative stress. This gene is structurally similar to the gene in the highly conserved redox-active disulfide bridge (Cys114, Cys236) and other highly conserved regions. In the present study, the gene was cloned into rice plants and PCC 7942 strain to study hydrogen peroxide (HO) stress responses. The gene expression was confirmed using semi-quantitative RT-PCR and western blot analysis. The gene expression increased growth under oxidative stress by decreasing reactive oxygen species and malondialdehyde level. Additionally, the gene expression in PCC 7942 (OT) strain exhibited a reduced loss of chlorophyll and enhanced photosynthesis efficiency under HO stress, thereby increasing biomass yields twofold compared with that of the control wild type (WT) strain. Furthermore, redox balance, ion homeostasis, molecular chaperone, and photosynthetic systems showed upregulation of some genes in the OT strain than in the WT strain by RNA-Seq analysis. Thus, gene expression enhances oxidative stress tolerance by increasing cell defense regulatory networks through the cellular redox homeostasis in the rice plants and PCC 7942.
蓝藻双半胱氨酸过氧化物酶（硫氧还蛋白过氧化物酶）是一类硫醇抗氧化酶家族，在氧化应激下对细胞存活至关重要。在我们之前的研究中，通过对遭受氧化应激的水稻（L.，）幼苗进行蛋白质组学分析，鉴定出了一个假定的。该基因在高度保守的氧化还原活性二硫键（Cys114，Cys236）和其他高度保守区域在结构上与基因相似。在本研究中，将基因克隆到水稻植株和集胞藻PCC 7942菌株中，以研究过氧化氢（HO）胁迫反应。使用半定量RT-PCR和蛋白质免疫印迹分析确认了基因表达。基因表达通过降低活性氧和丙二醛水平，在氧化应激下促进了生长。此外，集胞藻PCC 7942（OT）菌株中的基因表达在HO胁迫下表现出叶绿素损失减少和光合效率提高，从而使生物量产量比对照野生型（WT）菌株提高了两倍。此外，通过RNA测序分析，氧化还原平衡、离子稳态、分子伴侣和光合系统显示OT菌株中一些基因的上调程度高于WT菌株。因此，基因表达通过水稻植株和集胞藻PCC 7942中的细胞氧化还原稳态增加细胞防御调节网络，从而增强了氧化应激耐受性。
